ArticlePlant methods2017
Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress.
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Genome-Wide Identification and Characterization of Thaumatin-Like Proteins in Potato (Solanum tuberosum L.) and Their Role in Stress Tolerance.Molecular biotechnology · 2026Article
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Comprehensive genome-wide analysis of DUF668 gene family in potato reveals roles in growth and stress responses.Journal of applied genetics · 2026Article
- Arbuscular Mycorrhizal Fungi Modulate Variety-Specific Phosphate Transporter Gene Expression in Aerobic Rice Under Phosphorus-Limited Soil Conditions.Plants (Basel, Switzerland) · 2026Article
- Influence of cell-free culture supernatant and intact cell of endophytic bacteria on virulence traits of Ralstonia solanacearum, potato bacterial wilt agent.Scientific reports · 2026Article
- Proteomic Analysis of Lotus-DerivedPlants (Basel, Switzerland) · 2026Article
- Combined SMRT and Illumina RNA Sequencing Reveals the Alternative Splicing-Mediated Regulation of Anthocyanin Accumulation in Potato (Plants (Basel, Switzerland) · 2026Article
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Integrated multi-omics elucidates StAN1 as a key regulator reprogramming flavonoid partitioning and co-regulatory networks in potato tubers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- A comprehensive analysis to speculate the role of trithorax genes in photoperiod-mediated tuberization in potato and comparison with storage root crops.BMC plant biology · 2025Article
- qPCR-Based Reference Gene Validation inCurrent issues in molecular biology · 2025Article
- Selection of reference genes for normalization of mitochondrial gene expression by qRT-PCR in different potato tissues and during anther development.BMC plant biology · 2025Article
- Long Noncoding RNAs as Emerging Regulators of Seed Development, Germination, and Senescence.International journal of molecular sciences · 2025Review
- Stabilizing the Baseline: Reference Gene Evaluation in Three InvasiveInternational journal of molecular sciences · 2025Article
- The potential role of short internodes/stylish (SHI/STY) and SHI related sequence (SRS) in potato tuberization.BMC plant biology · 2025Article
- Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato.BMC genomics · 2025Article
- In-silico characterization and expression study of eIF genes associated with abiotic stresses in potato (Solanum tuberosum L.).Scientific reports · 2025Article
- Article
- Genome-Wide Identification of the Potato GGPS Gene Family and Analysis of Its Response to Abiotic Stress.Genes · 2025Article
- Transcriptome Profiling Identifies Key Regulators of Tuber Skin Color in Potato.Plants (Basel, Switzerland) · 2025Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReal-time quantitative PCR (RT-qPCR) is the most commonly used method for accurately detecting gene expression patterns. As part of RT-qPCR analysis, normalization of the data requires internal control gene(s) that display uniform expression under different biological conditions. However, no invariable internal control gene exists, and therefore more than one reference gene is needed to normalize RT-qPCR results. Identification of stable reference genes in potato will improve assay accuracy for selecting stress-tolerance genes and identifying molecular mechanisms conferring stress tolerance in this species.
resultsIn the experiment, we assessed the expression of eight candidate internal control genes, namely elongation factor-1alpha (
conclusionsThe results indicated that
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