Evidence map›Paper›PMID 29074968›Full record

ArticleNature communications2017

Coding and noncoding landscape of extracellular RNA released by human glioma stem cells.

Zhiyun Wei, Arsen O Batagov, Sergio Schinelli, Jintu Wang, Yang Wang, Rachid El Fatimy, Rosalia Rabinovsky, Leonora Balaj, Clark C Chen, Fred Hochberg and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 350 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
350citing papers in PubMed, 1 pooled it
19.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

350 citing papers in PubMed, 1 synthesis or guideline pooled it, 547 citations in OpenAlex.

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290 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 4 countries.

Zhiyun WeiDepartment of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.ORCID http://orcid.org/0000-0002-3554-4142
Arsen O BatagovVishuo Biomedical, #3-33 Teletech Park, 20 Science Park Road, Singapore, 117674, Singapore.
Sergio SchinelliDepartment of Drug Sciences, University of Pavia, Pavia, 27100, Italy.
Jintu WangBeijing Genomics Institute, Shenzhen, 518083, China.
Yang WangDepartment of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
Rachid El FatimyDepartment of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
Rosalia RabinovskyDepartment of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
Leonora BalajDepartment of Neurology and Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Charlestown, MA, 02129, USA.
Clark C ChenNeurosurgery Department, University of Minnesota, Minneapolis, MN, 55455, USA.
Fred HochbergDepartment of Neurosurgery, University of California, La Jolla, San Diego, CA, 92093, USA.
Bob CarterDepartment of Neurosurgery, University of California, La Jolla, San Diego, CA, 92093, USA.
Xandra O BreakefieldDepartment of Neurology and Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Charlestown, MA, 02129, USA.
Anna M KrichevskyDepartment of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA. akrichevsky@bwh.harvard.edu.
Brigham and Women's Hospital · USHarvard University · USUniversity of California San Diego · USBGI Group (China) · CNUniversity of Minnesota · USUniversity of Pavia · ITVishuo Biomedical (Singapore) · SG

Funding

The role of microvesicles in giloma virotherapyP01CA069246 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI CHAREST, ALAIN · 1996 to 2021
$30.9M
Transfer of microRNA regulators from glioblastoma to brain microenvironment U19CA179563 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI BREAKEFIELD, XANDRA OWENS, CHAREST, ALAIN · 2013 to 2017
$8.6M
Angiogenin and its small RNA products in glioblastoma and brain microenvironmentR21NS098051 · NINDS · BRIGHAM AND WOMEN'S HOSPITAL · PI KRICHEVSKY, ANNA M. · 2016 to 2017
$483k
NCI NIH HHS P01 CA069246NCI NIH HHS U19 CA179563NINDS NIH HHS R21 NS098051
6 · The paper itself

Abstract

Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, such as miRNAs in exosomes, and precisely processed tRNA and Y RNA fragments in EVs and exRNPs. EV-enclosed mRNAs are mostly fragmented, and UTRs enriched; nevertheless, some full-length mRNAs are present. Overall, there is less than one copy of non-rRNA per EV. Our results suggest that massive EV/exRNA uptake would be required to ensure functional impact of transferred RNA on brain recipient cells and predict the most impactful miRNAs in such conditions. This study also provides a catalog of diverse exRNAs useful for biomarker discovery and validates its feasibility on cerebrospinal fluid.

Indexed as

AnimalsBrain NeoplasmsCells, CulturedExtracellular VesiclesGene Expression Regulation, NeoplasticGliomaHumansMice, Inbred C57BLMicroRNAsNeoplastic Stem CellsRNA, MessengerRNA, NeoplasmRNA, UntranslatedTranscriptomeTumor Cells, CulturedMicroRNAsRNA, MessengerRNA, NeoplasmRNA, Untranslated

Identifiers

PMID29074968
PMCPMC5658400
OpenAlexW2766975063

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.