ArticleAnalytical chemistry2017
Microfluidic System for Detection of Viral RNA in Blood Using a Barcode Fluorescence Reporter and a Photocleavable Capture Probe.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment.Mechanobiology in medicine · 2024Review
- Micropillar enhanced FRET-CRISPR biosensor for nucleic acid detection.Lab on a chip · 2023Article
- Aryl-diazonium salts offer a rapid and cost-efficient method to functionalize plastic microfluidic devices for increased immunoaffinity capture.Advanced materials technologies · 2023Article
- Understanding the dynamics of fluid-structure interaction with an Air Deflected Microfluidic Chip (ADMC).Scientific reports · 2022Article
- An investigation into simplifying total RNA extraction with minimal equipment using a low volume, electrokinetically driven microfluidic protocol.Biomicrofluidics · 2022Article
- Fast custom wavelet analysis technique for single molecule detection and identification.Nature communications · 2022Article
- Review
- Smart materials-integrated sensor technologies for COVID-19 diagnosis.Emergent materials · 2021Review
- Microfluidic devices for detection of RNA viruses.Reviews in medical virology · 2021Review
- Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection System for Viral DNA Sensing.ACS omega · 2020Article
- 7X multiplexed, optofluidic detection of nucleic acids for antibiotic-resistance bacterial screening.Optics express · 2020Article
- 3× multiplexed detection of antibiotic resistant plasmids with single molecule sensitivity.Lab on a chip · 2020Article
- Advanced "lab-on-a-chip" to detect viruses - Current challenges and future perspectives.Biosensors & bioelectronics · 2020Review
- Perspective of Molecular Diagnosis in Healthcare: From Barcode to Pattern Recognition.Diagnostics (Basel, Switzerland) · 2019Article
- Integration of sample preparation and analysis into an optofluidic chip for multi-target disease detection.Lab on a chip · 2018Article
- Automated Optofluidic Analysis of Single Extracellular Vesicles for Organoid Culture Monitoring.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
A microfluidic sample preparation multiplexer (SPM) and assay procedure is developed to improve amplification-free detection of Ebola virus RNA from blood. While a previous prototype successfully detected viral RNA following off-chip RNA extraction from infected cells, the new device and protocol can detect Ebola virus in raw blood with clinically relevant sensitivity. The Ebola RNA is hybridized with sequence specific capture and labeling DNA probes in solution and then the complex is pulled down onto capture beads for purification and concentration. After washing, the captured RNA target is released by irradiating the photocleavable DNA capture probe with ultraviolet (UV) light. The released, labeled, and purified RNA is detected by a sensitive and compact fluorometer. Exploiting these capabilities, a detection limit of 800 attomolar (aM) is achieved without target amplification. The new SPM can run up to 80 assays in parallel using a pneumatic multiplexing architecture. Importantly, our new protocol does not require time-consuming and problematic off-chip probe conjugation and washing. This improved SPM and labeling protocol is an important step toward a useful POC device and assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.