ArticlePloS one2017
Transcriptome-wide analysis reveals the progress of Cordyceps militaris subculture degeneration.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Analysis of the Subculture Effect on theJournal of fungi (Basel, Switzerland) · 2026Article
- Parallel and comparative transcriptome and proteome provide insight into degeneration-related key candidates in Agaricus bisporus.BMC genomics · 2026Article
- Transcriptomic and Physiological Profiling Reveals Metabolic Determinants and Key Regulatory Hubs of Fruiting Body Degeneration inJournal of fungi (Basel, Switzerland) · 2026Article
- Accumulation of the spontaneous and random mutations is causative of fungal culture degeneration.Fundamental research · 2026Article
- A Comprehensive Characterisation of Mycelium-Based Biomaterials Developed fromJournal of fungi (Basel, Switzerland) · 2025Article
- A Review of Genomic, Transcriptomic, and Proteomic Applications in Edible Fungi Biology: Current Status and Future Directions.Journal of fungi (Basel, Switzerland) · 2025Review
- Integrated Transcriptomics and Metabolomics Provide Insight into Degeneration-Related Molecular Mechanisms ofJournal of fungi (Basel, Switzerland) · 2025Article
- Comparative transcriptomics and metabolomics provide insight into degeneration-related physiological mechanisms of Morchella importuna after long-term preservation.Microbial biotechnology · 2025Article
- Molecular Mechanism During Mycelium Subculture Degeneration ofJournal of fungi (Basel, Switzerland) · 2024Article
- Article
- Metabolomics Combined with Physiology and Transcriptomics Reveal the Response ofAntioxidants (Basel, Switzerland) · 2024Article
- A Review Delving into the Factors Influencing Mycelium-Based Green Composites (MBCs) Production and Their Properties for Long-Term Sustainability Targets.Biomimetics (Basel, Switzerland) · 2024Review
- Molecular mechanisms underlying phenotypic degeneration in Cordyceps militaris: insights from transcriptome reanalysis and osmotic stress studies.Scientific reports · 2024Article
- Antitumor Mechanism and Therapeutic Potential of Cordycepin Derivatives.Molecules (Basel, Switzerland) · 2024Article
- Article
- Effects ofJournal of fungi (Basel, Switzerland) · 2023Article
- The phenomenon of strain degeneration in biotechnologically relevant fungi.Applied microbiology and biotechnology · 2023Review
- Oxidative damage from repeated tissue isolation for subculturing causes degeneration inFrontiers in microbiology · 2023Article
- Nondestructive detection ofFrontiers in plant science · 2023Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe entomopathogenic mushroom Cordyceps militaris is an important medicinal and food resource owing to its various medicinal components and pharmacological effects. However, the high frequency of strain degeneration during subculture seriously restricts the large-scale production of C. militaris, and the mechanism underlying strain degeneration remains unclear. In this study, we artificially cultured C. militaris for six generations and compared changes during fruiting body growth. The transcriptome of six generations of C. militaris strains were sequenced with the Illumine Hiseq4000.
resultsThe subcultured C. militaris strains degenerated beginning at the third generation, with incomplete fruiting body growth beginning at the fourth generation. Over 9,015 unigenes and 731 new genes were identified. In addition, 35,323 alternative splicing (AS) events were detected in all samples, and more AS events occurred in the second, fourth and sixth generations. Compared with the first generation, the third generation (degenerated strain) included 2,498 differentially expressed genes (DEGs) including 1,729 up-regulated and 769 down-regulated genes. This number was higher than the number of DEGs in the second (1,892 DEGs), fourth (2,006 DEGs), fifth (2,273 DEGs) and sixth (2,188 DEGs) generations. Validation of RNA-seq by qRT-PCR showed that the expression patterns of 51 DEGs were in accordance with the transcriptome data.
conclusionOur results suggest that the mechanism of C. militaris strain degeneration is associated with gene involved in toxin biosynthesis, energy metabolism, and DNA methylation and chromosome remodeling.
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