Evidence map›Paper›PMID 29071818›Full record

ArticleAnnals of laboratory medicine2018

Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus subtyping.

Jaehyeon Lee, Hye Soo Lee, Yong Gon Cho, Sam Im Choi, Dal Sik Kim

Abstract readEvaluation Study
In one paragraph

Article in Annals of laboratory medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Cepheid XpertInfluenza and other respiratory viruses · 2021
    Article
  6. Article
  7. Article
  8. Characteristics of human parainfluenza virus type 4 infection in hospitalized children in Korea.Pediatrics international : official journal of the Japan Pediatric Society · 2020
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jaehyeon LeeDepartment of Laboratory Medicine, Chonbuk National University Hospital, Jeonju, Korea.
Hye Soo LeeDepartment of Laboratory Medicine, Chonbuk National University Medical School, Jeonju, Korea.
Yong Gon ChoDepartment of Laboratory Medicine, Chonbuk National University Medical School, Jeonju, Korea.
Sam Im ChoiDepartment of Laboratory Medicine, Chonbuk National University Medical School, Jeonju, Korea.
Dal Sik KimDepartment of Laboratory Medicine, Chonbuk National University Medical School, Jeonju, Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Allplex Respiratory Panel 1/2/3 (All16) is a multiplex PCR assay for detecting 16 respiratory viruses with influenza A virus (FluA) subtyping, and the first clinical assay based on multiple detection temperatures. We compared the results between All16 and Anyplex II RV16 (Any16) in 426 clinical samples. Samples showing discrepancies between the two tests were further tested using monoplex PCR. FluA subtyping based on the hemagglutinin type results of All16, which yielded H1, H3, and non-H1/H3, was compared with the results of the BioFire FilmArray respiratory panel. The positive and negative percent agreements and kappa value for each virus between All16 and Any16 ranged from 54.5-100.0%, 84.7-100.0%, and 0.57-1.00, respectively. FluA subtype results from All16 for 26 samples were consistent with those from FilmArray. Good agreement was observed between the two methods, except when analyzing human enterovirus (kappa value 0.70), and the All16 showed reliable FluA subtyping results. For parainfluenza virus 3, the All16 was more sensitive than Any16. When testing 28 samples simultaneously, the mean test time and hands-on time were 4.3 and 0.5 hours, respectively in All16. In conclusion, All16 showed reliable performance, but further studies are needed regarding human enterovirus analysis.

Indexed as

AdolescentAdultAgedAged, 80 and overChildChild, PreschoolHumansInfantInfant, NewbornInfluenza A virusMiddle AgedMultiplex Polymerase Chain ReactionNasopharynxReagent Kits, DiagnosticReal-Time Polymerase Chain ReactionRespiratory Tract InfectionsReagent Kits, DiagnosticRNA, ViralEvaluationInfluenza A subtypingMultiplex real-time PCRRespiratory VirusReverse transcriptase-PCR

Identifiers

PMID29071818
PMCPMC5700146

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.