Evidence map›Paper›PMID 29071293›Full record

ArticleData in brief2017

Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I.

Sarah E Lang, Tamara K Stevenson, Tabea M Schatz, Brandon J Biesiadecki, Margaret V Westfall

Abstract read
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In one paragraph

Article in Data in brief, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors.

Sarah E LangDepartment of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA.
Tamara K StevensonDepartment of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA.
Tabea M SchatzDepartment of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA.
Brandon J BiesiadeckiDepartment of Physiology and Cell Biology and Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
Margaret V WestfallDepartment of Cardiac Surgery, University of Michigan, Ann Arbor, MI 48109, USA.

Funding

CELLULAR AND MOLECULAR BIOLOGY AT MICHIGANT32GM007315 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PUTHENVEEDU, MANOJKUMAR A · 1985 to 2021
$12.8M
SYTEMS AND INTEGRATIVE BIOLOGYT32GM008322 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LOW, MALCOLM JAMES · 1990 to 2020
$5.7M
Integrated Crosstalk of Thin Filament Post-translational ModificationsR01HL114940 · NHLBI · OHIO STATE UNIVERSITY · PI BIESIADECKI, BRANDON J · 2013 to 2021
$3.6M
NHLBI NIH HHS R01 HL114940NIGMS NIH HHS T32 GM007315NIGMS NIH HHS T32 GM008322
6 · The paper itself

Abstract

Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western analysis was performed to determine whether the FLAG/total cTnI ratios are similar for phosphorylated versus total cTnI in myocytes expressing phospho-mimetic cTnI with Asp(D) substitutions at S43/45 plus S23/24 (cTnIS4D) or T144 (cTnISDTD). Representative Western analysis of phosphorylated S23/24 (p-S23/24) and S150 (p-S150) are presented along with re-probes using an antibody which detects all cTnI (MAB1691 Ab). The level of p-S150 also is compared to results obtained using single S43D and/or S45D phospho-mimetic substitutions. These results are discussed in more detail in Lang et al. [1].

Identifiers

PMID29071293
PMCPMC5651495

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.