Evidence map›Paper›PMID 29071182›Full record

ArticleNanotheranostics2017

Engineering Quantum Dots with Different Emission Wavelengths and Specific Fluorescence Lifetimes for Spectrally and Temporally Multiplexed Imaging of Cells.

Butian Zhang, Chengbin Yang, Yuan Gao, Yue Wang, Chengfei Bu, Siyi Hu, Liwei Liu, Hilmi Volkan Demir, Junle Qu, Ken-Tye Yong

Abstract read
In one paragraph

Article in Nanotheranostics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
  3. Theranostic Nanomedicine for Malignant Gliomas.Frontiers in bioengineering and biotechnology · 2019
    Review
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Butian ZhangSchool of Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore.
Chengbin YangSchool of Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore.
Yuan GaoSchool of Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore.
Yue WangChangchun University of Science and Technology, Changchun, 130022, P. R. China.
Chengfei BuChangchun University of Science and Technology, Changchun, 130022, P. R. China.
Siyi HuChangchun University of Science and Technology, Changchun, 130022, P. R. China.
Liwei LiuChangchun University of Science and Technology, Changchun, 130022, P. R. China.
Hilmi Volkan DemirSchool of Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore.
Junle QuKey Laboratory of Optoelectronics Devices and Systems of Ministry of Education /Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China.
Ken-Tye YongSchool of Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this work, a proof-of-concept study was performed to examine the potential of spectrally and temporally multiplexed imaging of cells by using quantum dots (QDs). The CdSe and ZAIS QDs with different emission wavelengths and well-separated fluorescence lifetimes were prepared to provide 2-dimensional information. After incubation with cells, the same type of QDs with different emission wavelengths were distinguishable in spectral imaging while different types of QDs with similar emission wavelengths but well-separated fluorescence lifetimes were resolvable in fluorescence lifetime imaging. For cells co-stained with dye and different types of QDs, the fluorescence lifetime imaging microscopy (FLIM) images showed spatially separated patterns that can be split into channel images by using the software-based time gates. Overall, the results demonstrate the feasibility of combining the 2-dimensional encoded QDs for spectrally and temporally multiplexed imaging. This method can be extended to other QDs and organic dyes to maximize the number of measurable species in multiplexed imaging and sensing applications.

Indexed as

Fluorescence LifetimeMultiplexed ImagingQuantum DotsTemporal Multiplexing.

Identifiers

PMID29071182
PMCPMC5646722

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.