ArticleScientific reports2017
New reverse genetics and transfection methods to rescue arboviruses in mosquito cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Reverse Genetics of Orthoflaviviruses: Strategies for Constructing Functional or Infectious cDNA.Journal of medical virology · 2026Review
- An alternative reverse genetics system for PRRS virus and its application to define the role of endocytic sorting signal in GP3 protein intracellular trafficking.Frontiers in microbiology · 2026Article
- West Nile virus vaccine candidates attenuated by dinucleotide enrichment are immunogenic and protective against lethal infection.PLoS pathogens · 2025Article
- Construction of an infectious clone of novel duck picornavirus using the infectious subgenomic amplicon method.Scientific reports · 2025Article
- Reverse genetics rescue of sylvatic dengue viruses.Journal of virology · 2025Article
- Infectious Subgenomic Amplicon Strategies for Japanese Encephalitis and West Nile Viruses.Journal of medical virology · 2025Article
- Infectivity in full-term placenta of Zika viruses with different lipid profiles.Virus research · 2025Article
- A simple reverse genetics method to generate recombinant coronaviruses.EMBO reports · 2022Article
- Replication is the key barrier during the dual-host adaptation of mosquito-borne flaviviruses.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- CpG content in the Zika virus genome affects infection phenotypes in the adult brain and fetal lymph nodes.Frontiers in immunology · 2022Article
- Facile method for delivering chikungunya viral replicons into mosquitoes and mammalian cells.Scientific reports · 2021Article
- Defective viral genomes as therapeutic interfering particles against flavivirus infection in mammalian and mosquito hosts.Nature communications · 2021Article
- Rapid Construction of an Infectious Clone of the Zika Virus, Strain ZKC2.Frontiers in microbiology · 2021Article
- Rolling circle amplification: A high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones.Virology · 2020Article
- Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells.Viruses · 2020Article
- CpG-Recoding in Zika Virus Genome Causes Host-Age-Dependent Attenuation of Infection With Protection Against Lethal Heterologous Challenge in Mice.Frontiers in immunology · 2019Article
- Live Zika virus chimeric vaccine candidate based on a yellow fever 17-D attenuated backbone.Emerging microbes & infections · 2018Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse genetics is a critical tool to decrypt the biological properties of arboviruses. However, whilst reverse genetics methods have been usually applied to vertebrate cells, their use in insect cells remains uncommon due to the conjunction of laborious molecular biology techniques and of specific difficulties surrounding the transfection of such cells. To leverage reverse genetics studies in both vertebrate and mosquito cells, we designed an improved DNA transfection protocol for insect cells and then demonstrated that the simple and flexible ISA (Infectious Subgenomic Amplicons) reverse-genetics method can be efficiently applied to both mammalian and mosquito cells to generate in days recombinant infectious positive-stranded RNA viruses belonging to genera Flavivirus (Japanese encephalitis, Yellow fever, West Nile and Zika viruses) and Alphavirus (Chikungunya virus). This method represents an effective option to potentially overcome technological issues related to the study of arboviruses.
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