ArticleScientific reports2017
Ecobody technology: rapid monoclonal antibody screening method from single B cells using cell-free protein synthesis for antigen-binding fragment formation.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Screening and machine learning-based prediction of translation-enhancing peptides that reduce ribosomal stalling inRSC chemical biology · 2026Article
- The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.Frontiers in bioengineering and biotechnology · 2026Review
- Identification of antibodies induced by immunization with the syphilis vaccine candidate Tp0751.Vaccine · 2025Article
- Development of Monoclonal Antibodies for Identifying Plant-Parasitic Nematodes.Journal of nematology · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Epitopes of an antibody that neutralizes a wide range of SARS-CoV-2 variants in a conserved subdomain 1 of the spike protein.Journal of virology · 2024Article
- A rapid cell-free expression and screening platform for antibody discovery.Nature communications · 2023Article
- Identification and Analysis of Monoclonal Antibodies with Neutralizing Activity against Diverse SARS-CoV-2 Variants.Journal of virology · 2023Article
- Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice.Science translational medicine · 2022Article
- Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multivalent designed proteins protect against SARS-CoV-2 variants of concern.bioRxiv : the preprint server for biology · 2021Article
- Evolution ofInternational journal of molecular sciences · 2020Review
- Rapid Generation of Monoclonal Antibodies from Single B Cells by Ecobody Technology.Antibodies (Basel, Switzerland) · 2018Article
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a rapid and cost-effective monoclonal antibody screening method from single animal B cells using reverse transcription (RT)-PCR and Escherichia coli cell-free protein synthesis (CFPS), which allows evaluation of antibodies within 2 working days. This process is named "Ecobody technology". The method includes strategies to isolate B cells that specifically bind an antigen from the peripheral blood of immunised animals, and single-cell RT-PCR to generate DNA fragments of the V
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.