ArticleParasites & vectors2017
Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
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Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 38 citations in OpenAlex.
- Diversity, geographical distribution, and prevalence of Entamoeba spp. in Brazil: a systematic review and meta-analysis.Parasite (Paris, France) · 2021Pooled it
- Prevalence of Blastocystis sp. infection in several hosts in Brazil: a systematic review and meta-analysis.Parasites & vectors · 2020Pooled it
- Emerging microbiome-directed therapies in inflammatory bowel disease: beyond diet modification and FMT.Seminars in immunopathology · 2025Review
- Discharges of Wastewater Treatment Plants Needed Further Monitoring to Minimize Potential Risk ofEnvironment & health (Washington, D.C.) · 2024Article
- Blastocystis colonization and associations with population parameters in Thai adults.PLoS neglected tropical diseases · 2024Article
- Molecular Prevalence and Subtypes Distribution ofTropical medicine and infectious disease · 2024Review
- Green synthesis of silver and iron oxide nanoparticles mediated photothermal effects on Blastocystis hominis.Lasers in medical science · 2024Article
- Diversity and composition of gut protist in young rural Zimbabwean children.Frontiers in microbiomes · 2024Article
- Distribution and Phylogenetic Analysis of Subtypes and Alleles of Blastocystis sp. in the Stool Samples Collected from Patients with Gastrointestinal Complaints in İzmir, Turkey.Acta parasitologica · 2023Article
- Phylogenetic relationship of nonmammalian and avianJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Enterobius vermicularis in Brazil: An integrative review.Revista da Sociedade Brasileira de Medicina Tropical · 2023Review
- An update on the distribution ofHeliyon · 2022Article
- Molecular characterization of Blastocystis subtypes isolated in the city of Uberaba, Minas Gerais State, Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2021Article
- Identification of MultipleFrontiers in veterinary science · 2021Article
- Article
- Determination of subtypes ofParasite epidemiology and control · 2020Article
- A Study on the Prevalence and Subtype Diversity of the Intestinal ProtistFrontiers in cellular and infection microbiology · 2020Article
- Culture isolation and molecular identification of Blastocystis sp. in Brazilian human isolates: preliminary results.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Characterization of subtypes ofParasite epidemiology and control · 2019Article
- Reduced prevalence of soil-transmitted helminths and high frequency of protozoan infections in the surrounding urban area of Curitiba, Paraná, Brazil.Parasite epidemiology and control · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil.
methodsA total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers.
resultsEndolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%).
conclusionsDNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.
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