ArticleLaboratory medicine2017
Leukocyte Dynamics Influence Reference Gene Stability in Whole Blood: Data-Driven qRT-PCR Normalization Is a Robust Alternative for Measurement of Transcriptional Biomarkers.
Article in Laboratory medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A comparison of normalization methods for the expression of genes associated with oxidative stress in the liver of sheep.BMC genomic data · 2025Article
- The Use of a Novel Container for Secured Transport and Storage of Biological Material for Quantitative Human RNA Analysis.International journal of molecular sciences · 2024Article
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- Male mouse skeletal muscle lacking HuR shows enhanced glucose disposal at a young age.Frontiers in physiology · 2024Article
- Variability in donor leukocyte counts confound the use of common RNA sequencing data normalization strategies in transcriptomic biomarker studies performed with whole blood.Scientific reports · 2023Article
- Rats lacking Ucp1 present a novel translational tool for the investigation of thermogenic adaptation during cold challenge.Acta physiologica (Oxford, England) · 2023Article
- Polyadenylate-binding protein cytoplasmic 1 mediates alternative splicing events of immune-related genes in gastric cancer cells.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- Use of deep artificial neural networks to identify stroke during triage via subtle changes in circulating cell counts.BMC neurology · 2022Article
- Use of high-sensitivity digital ELISA improves the diagnostic performance of circulating brain-specific proteins for detection of traumatic brain injury during triage.Neurological research · 2020Article
- Article
- Shifts in Leukocyte Counts Drive the Differential Expression of Transcriptional Stroke Biomarkers in Whole Blood.Translational stroke research · 2019Article
- Analysis of Early Stroke-induced Changes in Circulating Leukocyte Counts using Transcriptomic Deconvolution.Translational neuroscience · 2018Article
- Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke.Scientific reports · 2017Article
- Free Circulating miRNAs Measurement in Clinical Settings: The Still Unsolved Issue of the Normalization.Advances in clinical chemistryReview
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7 authors.
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Abstract
backgroundThe use of reference genes for normalization of whole blood qRT-PCR data may be problematic in conditions such as stroke which induce alterations in white blood cell differential. In this study, we assessed the influence of stroke on the stability of commonly employed reference genes, and we evaluated data-driven normalization as an alternative.
methodsPeripheral whole blood was sampled from 33 stroke patients and 29 controls, and qRT-PCR was used to measure the expression levels of 10 target genes whose transcripts are known stroke biomarkers. Target gene expression levels were normalized via those of 2 frequently cited reference genes (ACTB and B2M) as well as with the NORMA-Gene data-driven normalization algorithm.
resultsWhole blood expression levels of reference genes were significantly altered in stroke patients relative to controls. In comparison to normalization via reference genes, NORMA-Gene produced more robust target gene expression data in terms of differential expression dynamics, variance properties, and diagnostic performance.
conclusionsOur findings suggest that whole blood expression levels of commonly used reference genes may be sensitive to changes in white blood cell differential, and that data-driven qRT-PCR normalization approaches offer a powerful alternative.
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