ArticleBone2018
Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction.
Article in Bone, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Bone Multicellular Unit on a Chip (BMU-Chip) Subjected to Cyclic Mechanical Loading.ACS biomaterials science & engineering · 2026Article
- 3D Osteocyte Networks under Pulsatile Unidirectional Fluid Flow Stimuli (PUFFS).ACS biomaterials science & engineering · 2025Article
- Cellnet technology to generate 3D, functional, single-cell networks in custom architectures within collagen.Biofabrication · 2025Article
- Mapping the Response of Human Osteocytes in Native Matrix to Mechanical Loading Using RNA Sequencing.JBMR plus · 2023Article
- Review
- Mature primary human osteocytes in mini organotypic cultures secrete FGF23 and PTH1-34-regulated sclerostin.Frontiers in endocrinology · 2023Article
- Article
- Osteon: Structure, Turnover, and Regeneration.Tissue engineering. Part B, Reviews · 2022Review
- A Three-Dimensional Mechanical Loading Model of Human Osteocytes in Their Native Matrix.Calcified tissue international · 2022Article
- Microfluidic device engineered to study the trafficking of multiple myeloma cancer cells through the sinusoidal niche of bone marrow.Scientific reports · 2022Article
- Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research.Advanced healthcare materials · 2021Review
- Probing Osteocyte Functions in Gelatin Hydrogels with Tunable Viscoelasticity.Biomacromolecules · 2021Article
- Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques.Frontiers in endocrinology · 2021Review
- Perfused Platforms to Mimic Bone Microenvironment at the Macro/Milli/Microscale: Pros and Cons.Frontiers in cell and developmental biology · 2021Review
- A 3D, Dynamically Loaded Hydrogel Model of the Osteochondral Unit to Study Osteocyte Mechanobiology.Advanced healthcare materials · 2020Article
- Establishment ofExperimental and therapeutic medicine · 2020Article
- New and Old Osteocytic Cell Lines and 3D Models.Current osteoporosis reports · 2020Review
- IDG-SW3 Osteocyte Differentiation and Bone Extracellular Matrix Deposition Are Enhanced in a 3D Matrix Metalloproteinase-Sensitive Hydrogel.ACS applied bio materials · 2020Article
- Studies on Osteocytes in Their 3D Native Matrix Versus 2D In Vitro Models.Current osteoporosis reports · 2019Review
- Human ex vivo 3D bone model recapitulates osteocyte response to metastatic prostate cancer.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Osteocytes, residing as 3-dimensionally (3D) networked cells in bone, are well known to regulate bone and mineral homeostasis and have been recently implicated to interact with cancer cells to influence the progression of bone metastases. In this study, a bone tissue consisting of 3D-networked primary human osteocytes and MLO-A5 cells was constructed using: (1) the biomimetic close-packed assembly of 20-25μm microbeads with primary cells isolated from human bone samples and MLO-A5 cells and (2) subsequent perfusion culture in a microfluidic device. With this 3D tissue construction approach, we replicated ex vivo, for the first time, the mechanotransduction function of human primary osteocytes and MLO-A5 cells by correlating the effects of cyclic compression on down-regulated SOST and DKK1 expressions. Also, as an example of using our ex vivo model to evaluate therapeutic agents, we confirmed previously reported findings that parathyroid hormone (PTH) decreases SOST and increases the ratio of RANKL and OPG. In comparison to other in vitro models, our ex vivo model: (1) replicates the cell density, phenotype, and functions of primary human osteocytes and MLO-A5 cells and (2) thus provides a clinically relevant means of studying bone diseases and metastases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.