ArticleMicrobial cell factories2017
Mutants of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil as a biofactory for biodiesel production.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 79 citations in OpenAlex.
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- The use of droplet-based microfluidic technologies for accelerated selection ofBiology methods & protocols · 2024Review
- Biotechnological production of omega-3 fatty acids: current status and future perspectives.Frontiers in microbiology · 2023Review
- High-yield α-humulene production in Yarrowia lipolytica from waste cooking oil based on transcriptome analysis and metabolic engineering.Microbial cell factories · 2022Article
- Lipid production from lignocellulosic biomass using an engineered Yarrowia lipolytica strain.Microbial cell factories · 2022Article
- Physiological limitations and opportunities in microbial metabolic engineering.Nature reviews. Microbiology · 2022Review
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- Citrate-Mediated Acyl-CoA Synthesis Is Required for the Promotion of Growth and Triacylglycerol Production in Oleaginous YeastMicroorganisms · 2021Article
- Ethanol Production from Wheat Straw Hydrolysate by Issatchenkia Orientalis Isolated from Waste Cooking Oil.Journal of fungi (Basel, Switzerland) · 2021Article
- Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.MicrobiologyOpen · 2020Article
- Sustainable Production of Microbial Isoprenoid Derived Advanced Biojet Fuels Using Different Generation Feedstocks: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Effect of carbon source on lipid accumulation and biodiesel production of Yarrowia lipolytica.Environmental science and pollution research international · 2019Article
- Organic Wastes as Feedstocks for Non-Conventional Yeast-Based Bioprocesses.Microorganisms · 2019Review
- Utilization of Clarified Butter Sediment Waste as a Feedstock for Cost-Effective Production of Biodiesel.Foods (Basel, Switzerland) · 2019Article
- Bioreactor-Scale Strategies for the Production of Recombinant Protein in the YeastMicroorganisms · 2019Review
- Engineering the oleaginous yeastBiotechnology for biofuels · 2019Article
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOleaginous yeasts are fast emerging as a possible feedstock for biodiesel production. Yarrowia lipolytica, a model oleaginous yeast is known to utilize a variety of hydrophobic substrates for lipid accumulation including waste cooking oil (WCO). Approaches to increase lipid content in this yeast include metabolic engineering which requires manipulation of multiple genes in the lipid biosynthesis pathway. A classical and cost-effective approach, namely, random chemical mutagenesis on the yeast can lead to increased production of biodiesel as is explored here.
resultsIn this study, chemical mutagenesis using the alkylating agent, N- methyl-N'-nitro-N-nitrosoguanidine (MNNG) as well as an additional treatment with cerulenin, a fatty acid synthase inhibitor generated 800 mutants of Y. lipolytica NCIM 3589 (761 MNNG treated and 39 MNNG + cerulenin treated). A three-stage screening using Sudan Black B plate technique, Nile red fluorimetry and total lipid extraction using solvent was performed, which enabled selection of ten high lipid yielding mutants. Time course studies of all the ten mutants were further undertaken in terms of biomass, lipid yield and lipid content to select three stable mutants (YlB6, YlC7 and YlE1) capable of growing and accumulating lipid on WCO, with lipid contents of 55, 60 and 67% as compared to 45% for the wild type. The mutants demonstrated increased volumetric lipid productivities (0.062, 0.044 and 0.041 g L
conclusionThe chemical mutagenesis strategy adopted in this study resulted in the successful isolation of three stable high SCO yielding mutants. The mutants, namely, YlB6, YlC7 and YlE1 exhibited a 1.22, 1.33 and 1.49-fold increase in lipid contents when grown on 100 g L
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