ReviewMetabolites2017
Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols.
Review in Metabolites, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Marine microbiomes and their expanding role in biotechnological potential: a systematic review.Archives of microbiology · 2026Pooled it
- Application of Metabolomics in the Exploration of Bioactive Compounds and Drug Development from Medicinal Fungi.Metabolites · 2026Review
- Characterization of Secondary Metabolites from the Co-culture of Trichotomospora Caesia AC-1134 and Streptoverticillium sp. AC-1375 and Evaluation of its Antioxidant Properties.Current microbiology · 2026Article
- Screening of flavor-enhancing yeast and its role in fig fermentation.Frontiers in bioengineering and biotechnology · 2026Article
- Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage.Journal of visualized experiments : JoVE · 2025Article
- Metabolomic profiling of VOC-driven interactions between Priestia megaterium and Bacillus licheniformis in a simulated rhizosphere using split petri dishes.Archives of microbiology · 2025Article
- Exploring the Modulation of Extracellular Metabolites in DifferentFood science of animal resources · 2025Article
- Microbial Metabolites: A Sustainable Approach to Combat Plant Pests.Metabolites · 2025Review
- Developing a Cell Quenching Method to Facilitate Single Cell Mass Spectrometry Metabolomics Studies.JACS Au · 2025Article
- Investigation of different cold adaptation abilities in Salmonella enterica serotype Typhimurium strains using extracellular metabolomic approach.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Intracellular self-assembly and metabolite analysis of key enzymes for L-lysine synthesis based on key components of cellulosomes.Frontiers in microbiology · 2025Article
- Soil microbial resources: Unlocking sustainable strategies for crop productivity and soil health.Current research in microbial sciences · 2025Review
- Metabolic reprogramming in PGPR reveals cross-feeding-driven physiological shifts and metabolic adaptations.Frontiers in microbiology · 2025Article
- Unravelling lipid heterogeneity: Advances in single-cell lipidomics in cellular metabolism and disease.BBA advances · 2025Review
- Zic-HILIC MS/MS Method for NADomics Provides Novel Insights into Redox Homeostasis inMetabolites · 2024Article
- A systematic evaluation of quenching, extraction and analysis procedures for metabolomics study of the mechanism of QYSLD intervention in A549 cells.Analytical and bioanalytical chemistry · 2024Article
- Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.NPJ systems biology and applications · 2024Article
- Co-Culture of Gut Bacteria and Metabolite Extraction Using Fast Vacuum Filtration and Centrifugation.Methods and protocols · 2024Article
- Transcriptomic and metabolomic analysis of prebiotics utilization by Bifidobacterium animalis.World journal of microbiology & biotechnology · 2024Article
- Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sample preparation is one of the most important steps in metabolome analysis. The challenges of determining microbial metabolome have been well discussed within the research community and many improvements have already been achieved in last decade. The analysis of intracellular metabolites is particularly challenging. Environmental perturbations may considerably affect microbial metabolism, which results in intracellular metabolites being rapidly degraded or metabolized by enzymatic reactions. Therefore, quenching or the complete stop of cell metabolism is a pre-requisite for accurate intracellular metabolite analysis. After quenching, metabolites need to be extracted from the intracellular compartment. The choice of the most suitable metabolite extraction method/s is another crucial step. The literature indicates that specific classes of metabolites are better extracted by different extraction protocols. In this review, we discuss the technical aspects and advancements of quenching and extraction of intracellular metabolite analysis from microbial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.