ArticleLab on a chip2017
A simple culture system for long-term imaging of individual C. elegans.
Article in Lab on a chip, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Similarities and differences in the gene expression signatures of physiological age versus future lifespan.Aging cell · 2025Article
- Polyethylene glycol as an improved barrier to prevent fleeing inmicroPublication biology · 2024Article
- The million-molecule challenge: a moonshot project to rapidly advance longevity intervention discovery.GeroScience · 2023Article
- Adult-restricted gene knock-down reveals candidates that affect locomotive healthspan in C. elegans.Biogerontology · 2023Article
- High temporal resolution measurements of movement reveal novel early-life physiological decline in C. elegans.PloS one · 2022Article
- TheFrontiers in aging · 2022Article
- A polymer index-matched to water enables diverse applications in fluorescence microscopy.Lab on a chip · 2021Article
- Article
- Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies.Lab on a chip · 2020Article
- NemaLife chip: a micropillar-based microfluidic culture device optimized for aging studies in crawling C. elegans.Scientific reports · 2020Article
- The Detection of Early Epigenetic Inheritance of Mitochondrial Stress in C. Elegans with a Microfluidic Phenotyping Platform.Scientific reports · 2019Article
- Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms.Scientific reports · 2019Article
- Digging deeper: methodologies for high-content phenotyping in Caenorhabditis elegans.Lab animal · 2019Review
- Quantitative imaging of sleep behavior in Caenorhabditis elegans and larval Drosophila melanogaster.Nature protocols · 2019Article
- Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans.Lab on a chip · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have miniaturized standard culture techniques to rear arrays of isolated, individual C. elegans throughout their lives on solid gel media. The resulting apparatus is compatible with brightfield and fluorescence microscopy, enabling longitudinal studies of morphology and fluorescent transgene expression. Our culture system exploits a novel crosslinking reaction between a polyethylene glycol hydrogel and a silicone elastomer to constrain animals to individual "corrals" on the gel surface. These devices are simple to construct on the benchtop with commercially available reagents, and, unlike microfluidic isolation methods, do not rely on micropatterned materials. We demonstrate that this new culture method has negligible effects on the physiology of C. elegans compared to standard culture on agar plates. In addition, RNAi techniques are effective in this system. Finally, the hydrogel-silicone binding chemistry that we developed also allows traditional microfluidic devices to be covalently attached to gel substrates instead of glass.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.