ArticleNature communications2017
Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.Plant cell reports · 2026Review
- Stepwise construction of the path to doubled haploid breeding in sorghum.Science advances · 2026Article
- Grading evaluation of haploid fertility restoration traits based on inception-ResNet in maize.Plant phenomics (Washington, D.C.) · 2025Article
- Exploring potential strategies for haploid induction based on double fertilization in plants.Plant biotechnology journal · 2025Review
- Article
- Revolutionizing molecular plant breeding through genetic engineering of in vivo haploid induction genes.Molecular biology reports · 2025Review
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Harnessing haploid-inducer mediated genome editing for accelerated maize variety development.Plant biotechnology journal · 2025Article
- Karyotype variation patterns and phenotypic responses of hybrid progenies of triploid loquat (Horticulture research · 2025Article
- In vitro- and in vivo-based approaches for doubled haploid production in Zea mays L.: challenges and opportunities.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Phosphatidic acid signaling in modulating plant reproduction and architecture.Plant communications · 2025Review
- Perspectives of Genome Editing Mediated Haploid Inducer Systems in Legumes.International journal of molecular sciences · 2025Review
- Advances in doubled haploid technology for rice breeding: mechanisms, applications, and future perspectives.Frontiers in plant science · 2025Review
- Allelic variation and haplotype diversity ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- Genetic characteristics of the diploid offsprings in potato Cooperation 88 induced by diploid donor IVP101.Frontiers in plant science · 2024Article
- Engineering apomixis in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulation.Nature plants · 2023Article
- Synthetic apomixis enables stable transgenerational transmission of heterotic phenotypes in hybrid rice.Plant communications · 2023Article
- CRISPR/dCas-mediated gene activation toolkit development and its application for parthenogenesis induction in maize.Plant communications · 2023Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Production of maternal haploids using a conspecific haploid inducer is routine and highly efficient in maize. However, the underlying mechanism of haploid induction (HI) is unclear. We develop a method to isolate three nuclei from a pollen grain and four microspores from a tetrad for whole-genome sequencing. A high rate of aneuploidy is observed at the three-nucleus stage (6/22 pollens) rather than at the tetrad stage (1/72 microspores) in one HI line CAU5. Frequent aneuploidy is also observed in another two inducer lines, but not in two regular lines, which implies that HI may be associated with pollen aneuploidy. We further sequence the individual embryos and endosperms of 88 maize kernels crossing between regular and inducer lines. Genome-wide elimination of the CAU5-derived chromosome is identified in eight of 81 embryos. Together, these results suggest that continuous chromosome fragmentation occurring post meiosis in the gametophyte may cause haploidy of the embryo.
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