Evidence map›Paper›PMID 29058678›Full record

ArticleeLife2017

Computer assisted detection of axonal bouton structural plasticity in

Rohan Gala, Daniel Lebrecht, Daniela A Sahlender, Anne Jorstad, Graham Knott, Anthony Holtmaat, Armen Stepanyants

Abstract read
In one paragraph

Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Two-factor synaptic consolidation reconciles robustness with pruning and homeostatic scaling.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  11. Accurate registration ofProceedings of SPIE--the International Society for Optical Engineering · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rohan Gala *Department of Physics and Center for Interdisciplinary Research on Complex Systems, Northeastern University, Boston, United States.ORCID http://orcid.org/0000-0003-1872-0957
Daniel Lebrecht *Department of Basic Neurosciences, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Daniela A SahlenderBiological Electron Microscopy Facility, Centre of Electron Microscopy, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.ORCID http://orcid.org/0000-0002-0510-9529
Anne JorstadBiological Electron Microscopy Facility, Centre of Electron Microscopy, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.ORCID http://orcid.org/0000-0002-6438-1979
Graham KnottBiological Electron Microscopy Facility, Centre of Electron Microscopy, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.ORCID https://orcid.org/0000-0002-2956-9052
Anthony HoltmaatDepartment of Basic Neurosciences, Faculty of Medicine, University of Geneva, Geneva, Switzerland.ORCID http://orcid.org/0000-0002-7577-0769
Armen StepanyantsDepartment of Physics and Center for Interdisciplinary Research on Complex Systems, Northeastern University, Boston, United States.ORCID http://orcid.org/0000-0002-9387-2320

Funding

Software for Automated Reconstruction of Structure & Dynamics of Neural CircuitsR01NS091421 · NINDS · NORTHEASTERN UNIVERSITY · PI STEPANYANTS, ARMEN · 2015 to 2019
$1.8M
NINDS NIH HHS R01 NS091421
6 · The paper itself

Abstract

The ability to measure minute structural changes in neural circuits is essential for long-term in vivo imaging studies. Here, we propose a methodology for detection and measurement of structural changes in axonal boutons imaged with time-lapse two-photon laser scanning microscopy (2PLSM). Correlative 2PLSM and 3D electron microscopy (EM) analysis, performed in mouse barrel cortex, showed that the proposed method has low fractions of false positive/negative bouton detections (2/0 out of 18), and that 2PLSM-based bouton weights are correlated with their volumes measured in EM (

Indexed as

AnimalsImaging, Three-DimensionalIntravital MicroscopyMiceMicroscopy, ElectronMicroscopy, FluorescencePresynaptic TerminalsSomatosensory CortexTime-Lapse Imagingbouton detectionbouton volumeCLEMmouseneurosciencestructural plasticitysynaptic strengthtime-lapse imaging

Identifiers

PMID29058678
PMCPMC5675596

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.