Evidence map›Paper›PMID 29058209›Full record

ArticleJournal of endocrinological investigation2018

The rs2910164 variant is associated with reduced miR-146a expression but not cytokine levels in patients with type 2 diabetes.

B Alipoor, H Ghaedi, R Meshkani, M D Omrani, Z Sharifi, T Golmohammadi

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In one paragraph

Article in Journal of endocrinological investigation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 6 pooled it
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 6 syntheses or guidelines pooled it, 40 citations in OpenAlex.

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  19. Association of microRNA gene polymorphisms with Type 2 diabetes mellitus: A systematic review and meta-analysis.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 1 country.

B AlipoorDepartment of Laboratory Sciences, Faculty of Paramedicine, Yasuj University of Medical Sciences, Yasuj, Iran.
H GhaediDepartment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
R MeshkaniDepartment of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. rmeshkani@tums.ac.ir.
M D OmraniDepartment of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Z SharifiBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
T GolmohammadiDepartment of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. golmoham@sina.tums.ac.i.ORCID http://orcid.org/0000-0001-6388-6740
Shahid Beheshti University of Medical Sciences · IRHigh Institute for Education and Research in Transfusion Medicine · IRSina Hospital · IRTehran University of Medical Sciences · IRYasuj University of Medical Sciences · IR

Funding

Tehran University of Medical sciences 93-02-30-25172
6 · The paper itself

Abstract

purposePrevious reports have demonstrated that genetic variations in microRNAs regulome could affect microRNAs-mediated regulation. Therefore, in the present study we were aimed at (1) comparison of microRNA 146-a (miR-146a) peripheral blood mononuclear cells (PBMCs) and plasma levels between diabetic patients and controls, and (2) investigating the possible association of rs2910164 with miR-146a and its related target genes expression and also serum cytokine levels.

methodsThe study population consisted of 60 subjects including 30 type 2 diabetes (T2D) patients and 30 controls with determined genotypes for rs2910164. The RNA expression levels were determined by real-time PCR. Moreover, TNF-α, IL-6, IL-10 and IL-1β serum levels were measured using ELISA method.

resultsOur results showed that the miR-146a expression levels were significantly decreased in PBMCs (P = 0.004) and plasma (P = 0.008) samples of patients with T2D compared to healthy participants. In addition, we observed that IRAK1 mRNA expression-but not TLR4, TRAF6 and NFĸB-was significantly increased in patients with T2D compared to controls (P = 0.028). The relative expression levels of miR-146a in plasma and PBMCs samples of diabetic patients with the rs2910164 GG genotypes were significantly higher than that in CC (P < 0.05). Moreover, no significant differences were found in miR-146a targets and cytokine levels between the rs2910164 different genotypes.

conclusionOur study demonstrated that miR-146a circulating levels were significantly elevated in controls compared with T2D patients. In addition, we identified that rs2910164-C allele is associated with reduced expression levels of the miR-146a but not its mRNAs targets and cytokine levels in diabetic patients.

Indexed as

Polymorphism, Single NucleotideBiomarkersCase-Control StudiesCytokinesDiabetes Mellitus, Type 2FemaleFollow-Up StudiesGenetic Predisposition to DiseaseGenotypeHumansInterleukin-1 Receptor-Associated KinasesIntracellular Signaling Peptides and ProteinsLeukocytes, MononuclearMaleMicroRNAsMiddle AgedBiomarkersCytokinesInterleukin-1 Receptor-Associated KinasesIntracellular Signaling Peptides and ProteinsIRAK1 protein, humanMicroRNAsMIRN146 microRNA, humanNF-kappa BTifab protein, humanTLR4 protein, humanTNF Receptor-Associated Factor 6Toll-Like Receptor 4CytokinesDiabetesMicroRNAsmiR-146ars2910164

Identifiers

PMID29058209
OpenAlexW2766308966

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.