Evidence map›Paper›PMID 29058195›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies.

David J Posson, Radda Rusinova, Olaf S Andersen, Crina M Nimigean

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David J PossonDepartment of Anesthesiology, Weill Cornell Medical College, 1300 York Ave., New York, NY, 10065, USA.
Radda RusinovaDepartment of Physiology and Biophysics, Weill Cornell Medical College, 1300 York Ave., New York, NY, 10065, USA.
Olaf S AndersenDepartment of Physiology and Biophysics, Weill Cornell Medical College, 1300 York Ave., New York, NY, 10065, USA.
Crina M NimigeanDepartment of Anesthesiology, Weill Cornell Medical College, 1300 York Ave., New York, NY, 10065, USA. crn2002@med.cornell.edu.

Funding

Energetics of Channel-Bilayer InteractionsR01GM021342 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI ANDERSEN, OLAF S. · 1985 to 2021
$9.9M
Molecular Mechanisms of Potassium Channel Permeation and GatingR01GM088352 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI NIMIGEAN, CRINA M · 2010 to 2025
$5.6M
Gating Mechanisms of the Prokaryotic Calcium Activated Potassium channel MthKF32GM087865 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI POSSON, DAVID JOHN · 2009 to 2010
$109k
NIGMS NIH HHS F32 GM087865NIGMS NIH HHS R01 GM021342NIGMS NIH HHS R01 GM088352
6 · The paper itself

Abstract

Quantitative investigations into functional properties of purified ion channel proteins using standard electrophysiological methods are challenging, in particular for the determination of average ion channel behavior following rapid changes in experimental conditions (e.g., ligand concentration). Here, we describe a method for determining the functional activity of liposome-reconstituted K

Indexed as

AnimalsFatsFluorometryHumansLigand-Gated Ion ChannelsLipid BilayersLiposomesPotassium ChannelsFatsLigand-Gated Ion ChannelsLipid BilayersLiposomesPotassium ChannelstallowANTS quenchingIon channel functionLiposomal ion flux assayStopped-flow assayThallium

Identifiers

PMID29058195
PMCPMC5971093

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.