Evidence map›Paper›PMID 29058192›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Patch-Clamp Recordings of the KcsA K

Kimberly Matulef, Francis I Valiyaveetil

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kimberly MatulefProgram in Chemical Biology, Department of Physiology and Pharmacology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Mail Code L334, Portland, OR, 97239, USA.
Francis I ValiyaveetilDepartment of Physiology and Pharmacology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Mail Code L334, Portland, OR, 97239, USA. valiyave@ohsu.edu.
Oregon Health & Science University · US

Funding

Probing functional mechanisms in K+ channels using unnatural mutagenesisR01GM087546 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI VALIYAVEETIL, FRANCIS · 2009 to 2022
$4.0M
NIGMS NIH HHS R01 GM087546
6 · The paper itself

Abstract

Patch-clamp electrophysiology is the standard technique used for the high-resolution functional measurements on ion channels. While studies using patch clamp are commonly carried out following ion channel expression in a heterologous system such as Xenopus oocytes or tissue culture cells, these studies can also be carried out using ion channels reconstituted into lipid vesicles. In this chapter, we describe the methodology for reconstituting ion channels into liposomes and the procedure for the generation of unilamellar blisters from these liposomes that are suitable for patch clamp. Here, we focus on the bacterial K

Indexed as

BacteriaBacterial ProteinsElectrophysiologyLiposomesPatch-Clamp TechniquesPotassium ChannelsBacterial ProteinsLiposomesPotassium Channelsprokaryotic potassium channelBlistersGUV recordingsKcsALipid vesiclesPatch clamp

Identifiers

PMID29058192
PMCPMC6588281
OpenAlexW2765298509

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.