Evidence map›Paper›PMID 29058103›Full record

ArticlePlant molecular biology2017

Effects of transgenic expression of Brevibacterium linens methionine gamma lyase (MGL) on accumulation of Tylenchulus semipenetrans and key aminoacid contents in Carrizo citrange.

Elenor Castillo, Federico Martinelli, Florence Zakharov-Negre, Susan E Ebeler, Tom R Buzo, Michael V McKenry, Abhaya M Dandekar

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Elenor CastilloDepartment of Plant Sciences, University of California, Davis, 1 Shields Ave, Mail Stop 4, Davis, CA, 95616-8683, USA.
Federico MartinelliDipartimento di Scienze Agrarie Alimentari e Forestali, Università degli Studi di Palermo, Palermo, Italy.
Florence Zakharov-NegreDepartment of Plant Sciences, University of California, Davis, 1 Shields Ave, Mail Stop 4, Davis, CA, 95616-8683, USA.
Susan E EbelerDepartment of Viticulture and Enology, University of California, Davis, 1 Shields Ave, Davis, CA, 95616, USA.
Tom R BuzoDepartment of Nematology, University of California, Riverside, 900 University Ave, Riverside, CA, 92521, USA.
Michael V McKenryDepartment of Nematology, University of California, Riverside, 900 University Ave, Riverside, CA, 92521, USA.
Abhaya M DandekarDepartment of Plant Sciences, University of California, Davis, 1 Shields Ave, Mail Stop 4, Davis, CA, 95616-8683, USA. amdandekar@ucdavis.edu.
University of California, Davis · USUniversity of California, Riverside · USUniversity of Palermo · IT

Funding

Citrus Research Board 5300-138
6 · The paper itself

Abstract

key messageCarrizo transgenic plants overexpressing methionine-gamma-lyase produced dimethyl sulfide. The transgenic plants displayed more resistance to nematode attacks (Tylenculus semipenetrans) and may represent an innovative strategy for nematode control. Tylenchulus semipenetrans is a nematode pest of many citrus varieties that causes extensive damage to commercial crops worldwide. Carrizo citrange vr. (Citrus sinensis L. Usb × Poncirus trifoliate L. Raf) plants overexpressing Brevibacterium linens methionine-gamma-lyase (BlMGL) produced the sulfur volatile compound dimethyl sulfide (DMS). The aim of this work was to determine if transgenic citrus plants expressing BlMGL showed increased tolerance to T. semipenetrans infestation and to determine the effect on the content of key amino acids. While transgenic lines emitted dimethyl sulfide from leaves and roots, no sulfur-containing volatiles were detectable in wild-type Carrizo in the same tissues. Significant changes detected some key amino acids from leaves of transgenic plants such as aspartate, lysine, glycine, leucine and threonine with no changes in the amounts of methionine and α-ketobutyrate. In roots only glycine showed significant changes across all transgenic lines in comparison to wild-type plants. Transgenic plants expressing BlMGL and emitting DMS had less T. semipenetrans aggregation and more biomass than infected WT control plants, indicating that they may represent an innovative management alternative to pesticide/nematicide-based remedies.

Indexed as

Amino AcidsAnimalsBrevibacteriumCarbon-Sulfur LyasesCitrus sinensisMethioninePlant DiseasesPlant LeavesPlant RootsPlants, Genetically ModifiedSulfidesTylenchidaAmino AcidsCarbon-Sulfur Lyasesdimethyl sulfideL-methionine gamma-lyaseMethionineSulfidesBrevibacteriumMethionine gamma lyaseNematodesSulfur volatiles

Identifiers

PMID29058103
PMCPMC5688205
OpenAlexW2766901374

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.