ArticleScientific reports2017
Challenging FRET-based E-Cadherin force measurements in Drosophila.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Quantifying intracellular mechanosensitive response upon spatially defined mechano-chemical triggering.eLife · 2026Article
- Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs.Communications biology · 2026Article
- Paths to stability - actin regulation of adherens junction mechanics.Journal of cell science · 2025Review
- Three-dimensional traction technology and its application in mechanomedicine.Mechanobiology in medicine · 2025Review
- Expanding Horizons in Advancements of FRET Biosensing Technologies.Biosensors · 2025Review
- Genetically encoded mechano-sensors with versatile readouts and compact size.bioRxiv : the preprint server for biology · 2025Article
- Detection of fluorescent protein mechanical switching in cellulo.Cell reports methods · 2024Article
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- A comprehensive model ofeLife · 2023Article
- Mechanics of cell-cell junctions.Biophysical journal · 2023Article
- Engineering tools for quantifying and manipulating forces in epithelia.Biophysics reviews · 2023Review
- Spatiotemporal characterization of endothelial cell motility and physical forces during exposure toSTAR protocols · 2022Article
- Transgenic force sensors and software to measure force transmission across the mammalian nuclear envelope in vivo.Biology open · 2022Article
- Quantification of fast molecular adhesion by fluorescence footprinting.Science advances · 2021Article
- Volume measurement and biophysical characterization of mounds in epithelial monolayers after intracellular bacterial infection.STAR protocols · 2021Article
- Imaging methods in mechanosensing: a historical perspective and visions for the future.Molecular biology of the cell · 2021Review
- Bioimage Analysis and Cell Motility.Patterns (New York, N.Y.) · 2021Review
- Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology.Molecular biology of the cell · 2020Review
- Measuring mechanical stress in living tissues.Nature reviews. Physics · 2020Article
- Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical forces play a critical role during embryonic development. Cellular and tissue wide forces direct cell migration, drive tissue morphogenesis and regulate organ growth. Despite the relevance of mechanics for these processes, our knowledge of the dynamics of mechanical forces in living tissues remains scarce. Recent studies have tried to address this problem with the development of tension sensors based on Förster resonance energy transfer (FRET). These sensors are integrated into force bearing proteins and allow the measurement of mechanical tensions on subcellular structures. Here, we developed such a FRET-based sensor to measure E-Cadherin tensions in different Drosophila tissues in and ex vivo. Similar to previous studies, we integrated the sensor module into E-cadherin. We assessed the sensitivity of the sensor by measuring dynamic, developmental processes and mechanical modifications in three Drosophila tissues: the wing imaginal disc, the amnioserosa cells and the migrating border cells. However, these assays revealed that the sensor is not functional to measure the magnitude of tensions occurring in any of the three tissues. Moreover, we encountered technical problems with the measurement of FRET, which might represent more general pitfalls with FRET sensors in living tissues. These insights will help future studies to better design and control mechano-sensing experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.