Evidence map›Paper›PMID 29057887›Full record

ArticleScientific reports2017

Antimicrobial Peptides Secreted From Human Cryopreserved Viable Amniotic Membrane Contribute to its Antibacterial Activity.

Yong Mao, Tyler Hoffman, Anya Singh-Varma, Yi Duan-Arnold, Matthew Moorman, Alla Danilkovitch, Joachim Kohn

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 88 citations in OpenAlex.

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  19. Amnion membranes support wound granulation in a delayed murine excisional wound model.Clinical and experimental pharmacology & physiology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yong MaoNew Jersey Center for Biomaterials Rutgers University 145 Bevier Rd., Piscataway, NJ, 08854, United States.
Tyler HoffmanNew Jersey Center for Biomaterials Rutgers University 145 Bevier Rd., Piscataway, NJ, 08854, United States.
Anya Singh-VarmaNew Jersey Center for Biomaterials Rutgers University 145 Bevier Rd., Piscataway, NJ, 08854, United States.
Yi Duan-ArnoldOsiris Therapeutics, Inc, Columbia, MD, 21046, United States.
Matthew MoormanOsiris Therapeutics, Inc, Columbia, MD, 21046, United States.
Alla DanilkovitchOsiris Therapeutics, Inc, Columbia, MD, 21046, United States.
Joachim KohnNew Jersey Center for Biomaterials Rutgers University 145 Bevier Rd., Piscataway, NJ, 08854, United States. kohn@dls.rutgers.edu.
Rutgers, The State University of New Jersey · USOsiris Therapeutics (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic wounds remain a large problem in the field of medicine and are often associated with risk of infection and amputation. Recently, a commercially available human cryopreserved viable amniotic membrane (hCVAM) has been shown to effectively promote wound closure and reduce wound-related infections. A sprevious study indicates that hCVAM can inhibit the growth of bacteria associated with chronic wounds. In the present study, we investigated the mechanism of hCVAM antimicrobial activity. Our data demonstrate that antimicrobial activities against common pathogens in chronic wounds such as P.aeruginosa, S.aureus and Methicillin-resistant S.aureus (MRSA) are mediated via the secretion of soluble factors by viable cells in hCVAM and that these factors are proteins in nature. Further, we show that genes for antimicrobial peptides (AMPs) including human beta-defensins (HBDs) are expressed by hCVAM and that expression levels positively correlate with antimicrobial activity of hCVAM. At the protein level, our data indicate that HBD2 and HBD3 are secreted by hCVAM and directly contribute to its activity against P. aeruginosa. These data provide evidence that soluble factors including AMPs are hCVAM antimicrobial agents and are consistent with a role for AMPs in mediating antimicrobial properties of the membrane.

Indexed as

AmnionAnti-Bacterial AgentsAntimicrobial Cationic Peptidesbeta-DefensinsCryopreservationCulture Media, ConditionedCycloheximideDose-Response Relationship, DrugHumansMicrobial Sensitivity TestsProtein Synthesis InhibitorsPseudomonas aeruginosaPseudomonas InfectionsStaphylococcal InfectionsStaphylococcus aureusWound InfectionAnti-Bacterial AgentsAntimicrobial Cationic Peptidesbeta-DefensinsCulture Media, ConditionedCycloheximideDEFB4A protein, humanProtein Synthesis Inhibitors

Identifiers

PMID29057887
PMCPMC5651856
OpenAlexW2765151401

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.