Evidence map›Paper›PMID 29056478›Full record

ArticleResearch in microbiology2018

Survival of pathogenic Mycobacterium abscessus subsp. massiliense in Acanthamoeba castellanii.

Joas L da Silva, Jan Nguyen, Kevin P Fennelly, Adrian M Zelazny, Kenneth N Olivier

Abstract read
In one paragraph

Article in Research in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Frontiers in immunology · 2026
    Review
  3. Zebrafish larvae swim bladder serves as a model to studyMycobacteria (London, England) · 2026
    Article
  4. Article
  5. Virulence Mechanisms ofFrontiers in microbiology · 2022
    Review
  6. Article
  7. THP-1 andAntimicrobial agents and chemotherapy · 2019
    Article
  8. Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joas L da SilvaPulmonary Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Jan NguyenPulmonary Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Kevin P FennellyPulmonary Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Adrian M ZelaznyMicrobiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Kenneth N OlivierPulmonary Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address: kenneth.olivier@nih.gov.

Funding

Bronchiectasis and chronic airway infectionZIAHL006200 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI FENNELLY, KEVIN · 2015 to 2025
$25.1M
Molecular and Virulence Traits of Bacteria & Fungi Causing Persistent InfectionsZIACL080013 · CLC · CLINICAL CENTER · PI ZELAZNY, ADRIAN MARCELO · 2009 to 2025
$0k
Intramural NIH HHS Z99 HL999999Intramural NIH HHS ZIA HL006200
6 · The paper itself

Abstract

We used an amoeba model to study the intracellular growth and cytotoxicity of clinical strains of Mycobacterium abscessus subsp. massiliense (Mabsm) isolated from 2 patients (one with cystic fibrosis, the other one with idiopathic bronchiectasis) during the early (smooth colonies) and late stage (rough colonies) of chronic pulmonary infection. Acanthamoeba castellanii were infected with Mabsm (MOI 100) and samples collected every 24 h for 72 h. Results showed Mabsm is able to survive in trophozoites and persist in cysts for at least 7 days. Late Mabsm demonstrated higher cytotoxicity toward A. castellanii when compared to early strains. A. castellanii is a useful in vitro host model to study infection of Mabsm clinical isolates.

Indexed as

Microbial ViabilityAcanthamoeba castellaniiHumansModels, AnimalMycobacterium abscessusMycobacterium Infections, NontuberculousAcanthamoeba castellaniiChronic lung infectionMycobacterium abscessus

Identifiers

PMID29056478
PMCPMC5762252

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.