ArticleRedox biology2018
Cysteine perthiosulfenic acid (Cys-SSOH): A novel intermediate in thiol-based redox signaling?
Article in Redox biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Multifaceted roles for persulfide species in redox chemical biology.Nature chemical biology · 2026Review
- Raman signatures ofFrontiers in microbiology · 2026Article
- Metabolic dysfunction-associated steatohepatitis reduces hepatic HRedox biology · 2025Article
- Variant mutation G215C in SARS-CoV-2 nucleocapsid enhances viral infection via altered genomic encapsidation.PLoS biology · 2025Article
- Dual Roles of Reducing Systems in Protein Persulfidation and Depersulfidation.Antioxidants (Basel, Switzerland) · 2025Review
- Supersulfide donors and their therapeutic targets in inflammatory diseases.Frontiers in immunology · 2025Review
- Supersulphides provide airway protection in viral and chronic lung diseases.Nature communications · 2023Article
- Emerging Chemical Biology of Protein Persulfidation.Antioxidants & redox signaling · 2023Review
- The Effects of Antioxidant Nutraceuticals on Cellular Sulfur Metabolism and Signaling.Antioxidants & redox signaling · 2023Review
- Cysteine Oxidation in Proteins: Structure, Biophysics, and Simulation.Biochemistry · 2022Review
- Commonly Used Alkylating Agents Limit Persulfide Detection by Converting Protein Persulfides into Thioethers.Angewandte Chemie (International ed. in English) · 2022Article
- Chemical Biology of Reactive Sulfur Species: Hydrolysis-Driven Equilibrium of Polysulfides as a Determinant of Physiological Functions.Antioxidants & redox signaling · 2022Review
- Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and HMolecules (Basel, Switzerland) · 2021Article
- Cys-SH based quantitative redox proteomics of salt induced response in sugar beet monosomic addition line M14.Botanical studies · 2021Article
- Characterization of Endogenous and Extruded HACS chemical biology · 2021Article
- A Review of Chemical Tools for Studying Small Molecule Persulfides: Detection and Delivery.ACS chemical biology · 2021Review
- Regulation of the redox metabolome and thiol proteome by hydrogen sulfide.Critical reviews in biochemistry and molecular biology · 2021Article
- Comment on "Evidence that the ProPerDP method is inadequate for protein persulfidation detection due to lack of specificity".Science advances · 2021Article
- Can Blood-Circulating Factors Unveil and Delay Your Biological Aging?Biomedicines · 2020Review
- Sulfur Metabolism Under Stress.Antioxidants & redox signaling · 2020Article
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Authors and funding
12 authors.
Funding
Abstract
The reversible oxidation of protein cysteine residues (Cys-SH) is a key reaction in cellular redox signaling involving initial formation of sulfenic acids (Cys-SOH), which are commonly detected using selective dimedone-based probes. Here, we report that significant portions of dimedone-tagged proteins are susceptible to cleavage by DTT reflecting the presence of perthiosulfenic acid species (Cys-SSOH) due to similar oxidation of hydropersulfides (Cys-SSH), since Cys-S-dimedone adducts are stable toward DTT. Combined studies using molecular modeling, mass spectrometry, and cell-based experiments indicate that Cys-SSH are readily oxidized to Cys-SSOH, which forms stable adducts with dimedone-based probes. We additionally confirm the presence of Cys-SSH within protein tyrosine kinases such as EGFR, and their apparent oxidation to Cys-SSOH in response NADPH oxidase activation, suggesting that such Cys-SSH oxidation may represent a novel, as yet uncharacterized, event in redox-based signaling.
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