ArticlePloS one2017
3D morphological analysis of the mouse cerebral vasculature: Comparison of in vivo and ex vivo methods.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
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- Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imagingbioRxiv : the preprint server for biology · 2025Article
- Deficiency in N-cadherin-Akt3 signaling impairs the blood-brain barrier.Cell reports · 2025Article
- Depth-dependent contributions of various vascular zones to cerebral autoregulation and functional hyperemia: An in-silico analysis.PloS one · 2025Article
- Early Activation of Lung CD8Pathogens & immunity · 2025Article
- Non-invasive volumetric ultrasound localization microscopy detects vascular changes in mice with Alzheimer's disease.Theranostics · 2025Article
- The morphology of internal elastic lamina corrugations in arteries under physiological conditions.Morphologie : bulletin de l'Association des anatomistes · 2024Article
- Deep Mouse Brain Two-Photon Near-Infrared Fluorescence Imaging Using a Superconducting Nanowire Single-Photon Detector Array.ACS photonics · 2024Article
- Direct association with the vascular basement membrane is a frequent feature of myelinating oligodendrocytes in the neocortex.Fluids and barriers of the CNS · 2023Article
- The post-arteriole transitional zone: a specialized capillary region that regulates blood flow within the CNS microvasculature.The Journal of physiology · 2023Review
- Fluid transport in the brain.Physiological reviews · 2022Review
- Pericyte Control of Blood Flow Across Microvascular Zones in the Central Nervous System.Annual review of physiology · 2022Review
- Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature.International journal of molecular sciences · 2021Article
- Optical tissue clearing and machine learning can precisely characterize extravasation and blood vessel architecture in brain tumors.Communications biology · 2021Article
- CCR2 deficiency in monocytes impairs angiogenesis and functional recovery after ischemic stroke in mice.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- Microvascular Alterations in Alzheimer's Disease.Frontiers in cellular neuroscience · 2020Review
- Detection of Cerebrovascular Loss in the Normal Aging C57BL/6 Mouse Brain UsingFrontiers in aging neuroscience · 2020Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ex vivo 2-photon fluorescence microscopy (2PFM) with optical clearing enables vascular imaging deep into tissue. However, optical clearing may also produce spherical aberrations if the objective lens is not index-matched to the clearing material, while the perfusion, clearing, and fixation procedure may alter vascular morphology. We compared in vivo and ex vivo 2PFM in mice, focusing on apparent differences in microvascular signal and morphology. Following in vivo imaging, the mice (four total) were perfused with a fluorescent gel and their brains fructose-cleared. The brain regions imaged in vivo were imaged ex vivo. Vessels were segmented in both images using an automated tracing algorithm that accounts for the spatially varying PSF in the ex vivo images. This spatial variance is induced by spherical aberrations caused by imaging fructose-cleared tissue with a water-immersion objective. Alignment of the ex vivo image to the in vivo image through a non-linear warping algorithm enabled comparison of apparent vessel diameter, as well as differences in signal. Shrinkage varied as a function of diameter, with capillaries rendered smaller ex vivo by 13%, while penetrating vessels shrunk by 34%. The pial vasculature attenuated in vivo microvascular signal by 40% 300 μm below the tissue surface, but this effect was absent ex vivo. On the whole, ex vivo imaging was found to be valuable for studying deep cortical vasculature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.