ArticleBiochemistry2018
In Vivo Biochemistry: Single-Cell Dynamics of Cyclic Di-GMP in Escherichia coli in Response to Zinc Overload.
Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms.Analytical chemistry · 2024Article
- A label-free approach for relative spatial quantitation of c-di-GMP in microbial biofilms.bioRxiv : the preprint server for biology · 2023Article
- Sensory Perception in Bacterial Cyclic Diguanylate Signal Transduction.Journal of bacteriology · 2022Review
- Vibrio cholerae's mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and cell congregation.PLoS genetics · 2021Article
- Guanidine Biosensors Enable Comparison of Cellular Turn-on Kinetics of Riboswitch-Based Biosensor and Reporter.ACS synthetic biology · 2021Article
- Article
- Tracking the homeostasis of second messenger cyclic-di-GMP in bacteria.Biophysical reviews · 2020Review
- RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs.Current opinion in biotechnology · 2020Review
- Development of Ratiometric Bioluminescent Sensors forACS chemical biology · 2020Article
- Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP.Methods in enzymology · 2020Article
- Second messengers and divergent HD-GYP phosphodiesterases regulate 3',3'-cGAMP signaling.Molecular microbiology · 2020Article
- Cyclic-di-GMP Regulates Autoaggregation Through the Putative Peptidoglycan Hydrolase, EagA, and Regulates Transcription of theFrontiers in microbiology · 2020Article
- New Twists and Turns in Bacterial Locomotion and Signal Transduction.Journal of bacteriology · 2019Review
- "Second-generation" fluorogenic RNA-based sensors.Methods (San Diego, Calif.) · 2019Review
- Fluorescent Detection of the Ubiquitous Bacterial Messenger 3',5' Cyclic Diguanylic Acid by Using a Small Aromatic Molecule.Frontiers in microbiology · 2019Article
- Synthetic Biology of Small RNAs and Riboswitches.Microbiology spectrum · 2018Review
- Live Flow Cytometry Analysis of c-di-GMP Levels in Single Cell Populations.Methods in molecular biology (Clifton, N.J.) · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular signaling enzymes drive critical changes in cellular physiology and gene expression, but their endogenous activities in vivo remain highly challenging to study in real time and for individual cells. Here we show that flow cytometry can be performed in complex media to monitor single-cell population distributions and dynamics of cyclic di-GMP signaling, which controls the bacterial colonization program. These in vivo biochemistry experiments are enabled by our second-generation RNA-based fluorescent (RBF) biosensors, which exhibit high fluorescence turn-on in response to cyclic di-GMP. Specifically, we demonstrate that intracellular levels of cyclic di-GMP in Escherichia coli are repressed with excess zinc, but not with other divalent metals. Furthermore, in both flow cytometry and fluorescence microscopy setups, we monitor the dynamic increase in cellular cyclic di-GMP levels upon zinc depletion and show that this response is due to de-repression of the endogenous diguanylate cyclase DgcZ. In the presence of zinc, cells exhibit enhanced cell motility and increased sensitivity to antibiotics due to inhibited biofilm formation. Taken together, these results showcase the application of RBF biosensors in visualizing single-cell dynamic changes in cyclic di-GMP signaling in direct response to environmental cues such as zinc and highlight our ability to assess whether observed phenotypes are related to specific signaling enzymes and pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.