ArticleEnvironmental microbiology2017
Comprehensive functional characterization of the glycoside hydrolase family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification.
Article in Environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
- Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl-Glucose Phosphorylase and a Mannobiose Epimerase.Molecular microbiology · 2026Article
- Bio-inspired designer cellulosomes show strongest synergy on industrial substrates under natural-like conditions.Journal of industrial microbiology & biotechnology · 2026Article
- The physiological functions of the Cbp2D and Cbp2E proteins are important for insoluble cellulose-dependent growth inApplied and environmental microbiology · 2025Article
- A Multiplexing Activity-Based Protein-Profiling Platform for Dissection of a Native Bacterial Xyloglucan-Degrading System.ACS central science · 2023Article
- Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode.Applied microbiology and biotechnology · 2022Article
- Disentangling the genetic basis of rhizosphere microbiome assembly in tomato.Nature communications · 2022Article
- Biocatalytic quantification of α-glucan in marine particulate organic matter.MicrobiologyOpen · 2022Article
- Unifying themes and distinct features of carbon and nitrogen assimilation by polysaccharide-degrading bacteria: a summary of four model systems.Applied microbiology and biotechnology · 2021Review
- Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors.Nature communications · 2021Article
- Trehalose Degradation by Cellvibrio japonicus Exhibits No Functional Redundancy and Is Solely Dependent on the Tre37A Enzyme.Applied and environmental microbiology · 2020Article
- Laminarin is a major molecule in the marine carbon cycle.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Complete Genome Sequences of Cellvibrio japonicus Strains with Improved Growth When Using α-Diglucosides.Microbiology resource announcements · 2019Article
- Substrate specificity, regiospecificity, and processivity in glycoside hydrolase family 74.The Journal of biological chemistry · 2019Article
- Article
- Systems analysis of the glycoside hydrolase family 18 enzymes fromThe Journal of biological chemistry · 2018Article
- In vitro and in vivo characterization of threeBiotechnology for biofuels · 2018Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1→3)/β(1→4) mixed-linkage glucan (MLG) and β(1→3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has a robust capacity for plant polysaccharide degradation, due to a genome encoding a large contingent of Carbohydrate-Active enZymes (CAZymes), many of whose specific functions remain unknown. Using a comprehensive genetic and biochemical approach, we have delineated the physiological roles of the four C. japonicus glycoside hydrolase family 3 (GH3) members on diverse β-glucans. Despite high protein sequence similarity and partially overlapping activity profiles on disaccharides, these β-glucosidases are not functionally equivalent. Bgl3A has a major role in MLG and sophorose utilization, and supports β(1→3) glucan utilization, while Bgl3B underpins cellulose utilization and supports MLG utilization. Bgl3C drives β(1→3) glucan utilization. Finally, Bgl3D is the crucial β-glucosidase for XyG utilization. This study not only sheds the light on the metabolic machinery of C. japonicus, but also expands the repertoire of characterized CAZymes for future deployment in biotechnological applications. In particular, the precise functional analysis provided here serves as a reference for informed bioinformatics on the genomes of other Cellvibrio and related species.
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