Evidence map›Paper›PMID 29052144›Full record

ArticleMolecular neurobiology2018

The Effects of Extracellular Serum Concentration on APP Processing in Npc1-Deficient APP-Overexpressing N2a Cells.

M Maulik, D Vergote, G Phukan, J Chung, G Thinakaran, S Kar

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

M MaulikCentre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, Alberta, T6G 2M8, Canada.
D VergoteFaculté Saint-Jean, University of Alberta, Edmonton, Alberta, T6G 2M8, Canada.
G PhukanCentre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, Alberta, T6G 2M8, Canada.
J ChungCentre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, Alberta, T6G 2M8, Canada.
G ThinakaranDepartments of Neurobiology, Neurology, and Pathology, The University of Chicago, Chicago, IL, 60637, USA.
S KarCentre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, Alberta, T6G 2M8, Canada. skar@ualberta.ca.ORCID http://orcid.org/0000-0003-0167-3808
University of Alberta · CAIndian Institute of Science Education and Research Kolkata · INUniversity of Chicago · US

Funding

Alberta Innovates - Health Solutions 200900673Canadian Institutes of Health Research MOP-123258University of Alberta Hospital Foundation RES0029064
6 · The paper itself

Abstract

Amyloid precursor protein (APP) is cleaved by a set of proteases including α-/β-/γ- and recently identified η-secretases, generating C-terminal fragments (CTFs) of varying lengths and amyloid β (Aβ) peptides, which are considered to play a pivotal role in Alzheimer's disease (AD) pathogenesis. Cellular cholesterol content/distribution can regulate the production/clearance of APP metabolites and hence modify AD pathology. To determine the functional relation between endosomal-lysosomal (EL) cholesterol sequestration and APP metabolism, we used our recently developed mouse N2a-ANPC cells that overexpress Swedish mutant human APP in the absence of cholesterol-trafficking Niemann-Pick type C1 (Npc1) protein. Here, we report that neither increased levels nor EL cholesterol sequestration altered APP holoprotein levels but caused the intracellular accumulation of APP α-/β-/η-CTFs and Aβ

Indexed as

Amyloid beta-PeptidesAnimalsCell LineCell SurvivalCholesterolExtracellular SpaceMiceNiemann-Pick C1 ProteinSerumAmyloid beta-PeptidesCholesterolNiemann-Pick C1 ProteinAlzheimer’s diseaseAmyloid β peptideAPP processingCholesterol accumulationEndosomal-lysosomal systemNiemann-Pick type C1 disease

Identifiers

PMID29052144
OpenAlexW2765977089

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.