ArticleAIMS biophysics2017
Protein crystallization: Eluding the bottleneck of X-ray crystallography.
Article in AIMS biophysics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Shoebill: an interpretable AlphaFold2-informed predictor of protein crystallization propensity using XGBoost.Briefings in bioinformatics · 2026Article
- Research Progress on Sex Pheromone Receptors in Insects.Insects · 2026Review
- A comprehensive foundation model for cryo-EM image processing.Nature methods · 2026Article
- Using AlphaFold and Symmetrical Docking to Predict Protein-Protein Interactions for Exploring Potential Crystallization Conditions.Proteins · 2025Article
- Assignment-Free Determination of Ligand Binding Sites in Proteins by Solid-State NMR.Journal of the American Chemical Society · 2025Article
- Sensing Protein Structural Transitions with Microfluidic Modulation Infrared Spectroscopy.Biosensors · 2025Article
- Review
- Heterogeneous Nucleation in Protein Crystallization.Biomimetics (Basel, Switzerland) · 2023Review
- X-ray Crystallography: Seeding Technique with Cytochrome P450 Reductase.Bio-protocol · 2022Article
- Non-covalent Fc-Fab interactions significantly alter internal dynamics of an IgG1 antibody.Scientific reports · 2022Article
- Advances in antibody discovery from human BCR repertoires.Frontiers in bioinformatics · 2022Review
- Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry.Structure (London, England : 1993) · 2021Article
- Computational Approaches: An Underutilized Tool in the Quest to Elucidate Radical SAM Dynamics.Molecules (Basel, Switzerland) · 2021Review
- Studying the Structures of Relaxed and Fuzzy Interactions: The Diverse World of S100 Complexes.Frontiers in molecular biosciences · 2021Review
- In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices.International journal of molecular sciences · 2020Article
- Protein structural changes on a CubeSat under rocket acceleration profile.NPJ microgravity · 2020Article
- Archiving and disseminating integrative structure models.Journal of biomolecular NMR · 2019Article
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Authors and funding
6 authors.
Funding
Abstract
To date, X-ray crystallography remains the gold standard for the determination of macromolecular structure and protein substrate interactions. However, the unpredictability of obtaining a protein crystal remains the limiting factor and continues to be the bottleneck in determining protein structures. A vast amount of research has been conducted in order to circumvent this issue with limited success. No single method has proven to guarantee the crystallization of all proteins. However, techniques using antibody fragments, lipids, carrier proteins, and even mutagenesis of crystal contacts have been implemented to increase the odds of obtaining a crystal with adequate diffraction. In addition, we review a new technique using the scaffolding ability of PDZ domains to facilitate nucleation and crystal lattice formation. Although in its infancy, such technology may be a valuable asset and another method in the crystallography toolbox to further the chances of crystallizing problematic proteins.
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