ArticleJournal of tissue engineering
Making microenvironments: A look into incorporating macromolecular crowding into in vitro experiments, to generate biomimetic microenvironments which are capable of directing cell function for tissue engineering applications.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Understanding Osmolality as a Critical Process Parameter in Mammalian Muscle Cell Culture and Biomanufacturing.ACS omega · 2026Review
- Advances in cell-derived extracellular matrix for 3D tumor modelling.Materials today. Bio · 2026Review
- Serum-free culture with PVP enables maintenance of primary culture of parathyroid cells in vitro.Bioscience reports · 2026Article
- Harnessing the Biomimetic Effect of Macromolecular Crowding in the Cell-Derived Model of Clubfoot Fibrosis.Biomacromolecules · 2024Article
- Human amniotic membrane modulates collagen production and deposition in vitro.Scientific reports · 2024Article
- Hierarchical Design of Tissue-Mimetic Fibrillar Hydrogel Scaffolds.Advanced healthcare materials · 2024Review
- Article
- Adipose-derived stromal cells increase the formation of collagens through paracrine and juxtacrine mechanisms in a fibroblast co-culture model utilizing macromolecular crowding.Stem cell research & therapy · 2022Article
- Progress on Crowding Effect in Cell-like Structures.Membranes · 2022Review
- Subcellular Architecture of themBio · 2021Article
- Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET.Current topics in membranes · 2021Article
- Animal and Human Models of Tissue Repair and Fibrosis: An Introduction.Methods in molecular biology (Clifton, N.J.) · 2021Review
- The Scar-in-a-Jar: In Vitro Fibrosis Model for Anti-Fibrotic Drug Testing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- Macromolecular Crowding Enhances the Detection of DNA and Proteins by a Solid-State Nanopore.Nano letters · 2020Article
- Impact of crosslink heterogeneity on extracellular matrix mechanics and remodeling.Computational and structural biotechnology journal · 2020Article
- Macromolecular Crowding as a Tool to Screen Anti-fibrotic Drugs: The Scar-in-a-Jar System Revisited.Frontiers in medicine · 2020Article
- Fibrillar fibronectin plays a key role as nucleator of collagen I polymerization during macromolecular crowding-enhanced matrix assembly.Biomaterials science · 2019Article
- The extracellular fluid macromolecular composition differentially affects cell-substrate adhesion and cell morphology.Scientific reports · 2019Article
- A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts.BMC biomedical engineering · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomimetic microenvironments are key components to successful cell culture and tissue engineering in vitro. One of the most accurate biomimetic microenvironments is that made by the cells themselves. Cell-made microenvironments are most similar to the in vivo state as they are cell-specific and produced by the actual cells which reside in that specific microenvironment. However, cell-made microenvironments have been challenging to re-create in vitro due to the lack of extracellular matrix composition, volume and complexity which are required. By applying macromolecular crowding to current cell culture protocols, cell-made microenvironments, or cell-derived matrices, can be generated at significant rates in vitro. In this review, we will examine the causes and effects of macromolecular crowding and how it has been applied in several in vitro systems including tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.