Evidence map›Paper›PMID 29051621›Full record

ArticleScientific reports2017

Vector-independent transmembrane transport of oligodeoxyribonucleotides involves p38 mitogen activated protein kinase phosphorylation.

Minyuan Peng, Yanming Li, Jian Zhang, Yong Wu, Xiaoyang Yang, Ye Lei, Mao Ye, Jing Liu, Xu Han, Yijin Kuang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Minyuan PengHematology Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.ORCID 0000-0001-8173-3457
Yanming LiLaboratory Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Jian ZhangHematology Department, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
Yong WuHematology Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Xiaoyang YangHematology Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Ye LeiUrology surgery Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Mao YeMolecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, Hunan, China.
Jing LiuMolecular Biology Research Center, School of Life Science, Central South University, Changsha, 410078, China.
Xu HanMolecular Biology Research Center, School of Life Science, Central South University, Changsha, 410078, China.
Yijin KuangMolecular Biology Research Center, School of Life Science, Central South University, Changsha, 410078, China.
Xielan ZhaoHematology Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Fangping ChenHematology Department, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China. xychenfp@qq.com.
Central South University · CNHunan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The main roles of equilibrative nucleoside transporters (ENTs) and concentrative nucleoside transporters (CNTs) are to transfer single nucleosides and analogues for the nucleic acid salvage pathway. Oligodeoxyribonucleotides (ODNs) can be transported into the cytoplasm or nucleus of cells under certain conditions. Among ODNs composed of a single type of nucleotide, the transport efficiency differs with the length and nucleotide composition of the ODNs and varies in different types of leukaemia cells; among the 5 tested random sequence ODNs and 3 aptamers with varying sequences, the data showed that some sequences were associated with significantly higher transport efficiency than others. The transport of ODNs was sodium, energy, and pH-independent, membrane protein-dependent, substrate nonspecific for ODNs and 4-nitrobenzylthioinosine (NBMPR)-insensitive, but it showed a low sensitivity to dipyridamole (IC50 = 35.44 µmol/L), distinguishing it from ENT1-4 and CNTs. The delivery efficiency of ODNs was superior to that of Lipofection and Nucleofection, demonstrating its potential applications in research or therapeutics. Moreover, this process was associated with p38 mitogen activated protein kinase (p38MAPK) instead of c-Jun N-terminal kinase (JNK) signalling pathways. We have denoted ODN transmembrane transport as equilibrative nucleic acid transport (ENAT). Overall, these findings indicate a new approach and mechanism for transmembrane transport of ODNs.

Indexed as

AdolescentAdultAgedBiological TransportChildChild, PreschoolCytoplasmDipyridamoleFemaleHumansInfantK562 CellsLeukemiaMaleMiddle AgedOligodeoxyribonucleotides4-nitrobenzylthioinosineDipyridamoleOligodeoxyribonucleotidesp38 Mitogen-Activated Protein KinasesThioinosine

Identifiers

PMID29051621
PMCPMC5648841
OpenAlexW2761862902

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.