Evidence map›Paper›PMID 29051555›Full record

ArticleScientific reports2017

The first characterized phage against a member of the ecologically important sphingomonads reveals high dissimilarity against all other known phages.

Tue Kjærgaard Nielsen, Alexander Byth Carstens, Patrick Browne, René Lametsch, Horst Neve, Witold Kot, Lars Hestbjerg Hansen

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Novel LyticViruses · 2026
    Article
  2. Article
  3. New Bacteriophages Members of thePHAGE (New Rochelle, N.Y.) · 2023
    Article
  4. Article
  5. The First Cbk-Like Phage InfectingFrontiers in microbiology · 2022
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Tue Kjærgaard NielsenDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399C, 4000, Roskilde, Denmark.
Alexander Byth CarstensDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399C, 4000, Roskilde, Denmark.
Patrick BrowneDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399C, 4000, Roskilde, Denmark.
René LametschDepartment of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg C, Denmark.
Horst NeveDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Hermann-Weigmann-Straße 1, 24103, Kiel, Germany.
Witold KotDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399C, 4000, Roskilde, Denmark.
Lars Hestbjerg HansenDepartment of Environmental Science, Aarhus University, Frederiksborgvej 399C, 4000, Roskilde, Denmark. lhha@envs.au.dk.
Aarhus University · DKMax Rubner Institut · DEUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study describes the first molecular characterization of a bacteriophage infecting a member of the environmentally important Sphingomonadaceae family. Both bacteriophage Lacusarx and its host Sphingobium sp. IP1 were isolated from activated sludge from a wastewater treatment plant. Genome sequencing revealed that the phage genes display little similarity to other known phages, despite a remarkable conservation of the synteny in which the functional genes occur among distantly related phages. Phylogenetic analyses confirmed that Lacusarx represents a hitherto undescribed genus of phages. A classical lysis cassette could not be identified in Lacusarx, suggesting that the genes encoding endolysin, holin, and spanin are host-specific and not found in phages infecting other bacteria. The virus harbors 24 tRNA genes corresponding to 18 different amino acids and furthermore has a significantly different codon usage than its host. Proteomic analysis of Lacusarx revealed the protein components of the phage particle. A lysogeny test indicated that Lacusarx is not a temperate phage.

Indexed as

Bacterial ProteinsBacteriophagesEndopeptidasesGenome, ViralPhylogenyRNA, TransferSequence Analysis, DNASphingomonadaceaeWastewaterBacterial ProteinsendolysinEndopeptidasesRNA, TransferWastewater

Identifiers

PMID29051555
PMCPMC5648845
OpenAlexW2762329156

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.