ArticleScientific reports2017
The efficacy and further functional advantages of random-base molecular barcodes for absolute and digital quantification of nucleic acid molecules.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile.eLife · 2024Article
- High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ.Nature protocols · 2024Review
- Integration of single-cell manipulation, whole transcriptome analysis, and image-based deep learning for studying "Singularity Biology".Biophysics and physicobiology · 2024Article
- CD45 alleviates airway inflammation and lung fibrosis by limiting expansion and activation of ILC2s.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- High-throughput identification and quantification of single bacterial cells in the microbiota.Nature communications · 2022Article
- Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic mossFrontiers in plant science · 2022Article
- Sequencing the origins of life.BBA advances · 2022Review
- Development of a program for in silico optimized selection of oligonucleotide-based molecular barcodes.PloS one · 2021Article
- BSJ 2019 "Single-cell PRESTO" session.Biophysical reviews · 2020Article
- HaloTag-based conjugation of proteins to barcoding-oligonucleotides.Nucleic acids research · 2020Article
- Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity.Nature communications · 2020Article
- Cbfβ2 controls differentiation of and confers homing capacity to prethymic progenitors.The Journal of experimental medicine · 2018Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate quantification of biomolecules in system-wide measurements is in high demand, especially for systems with limited sample amounts such as single cells. Because of this, digital quantification of nucleic acid molecules using molecular barcodes has been developed, making, e.g., transcriptome analysis highly reproducible and quantitative. This counting scheme was shown to work using sequence-restricted barcodes, and non-sequence-restricted (random-base) barcodes that may provide a much higher dynamic range at significantly lower cost have been widely used. However, the efficacy of random-base barcodes is significantly affected by base changes due to amplification and/or sequencing errors and has not been investigated experimentally or quantitatively. Here, we show experimentally that random-base barcodes enable absolute and digital quantification of DNA molecules with high dynamic range (from one to more than 10
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