ArticleArteriosclerosis, thrombosis, and vascular biology2018
Mining the Stiffness-Sensitive Transcriptome in Human Vascular Smooth Muscle Cells Identifies Long Noncoding RNA Stiffness Regulators.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Female-Biased VSMC GRNs Predict MYH9 as Regulator of Fibrous Plaque Phenotype.Circulation research · 2026Article
- Targeting PDGF/PDGFR Signaling Pathway by microRNA, lncRNA, and circRNA for Therapy of Vascular Diseases: A Narrow Review.Biomolecules · 2024Review
- Long non-coding RNA MALAT1 sponges miR-30c to promote the calcification of human vascular smooth muscle cells by regulating Runx2.Renal failure · 2023Article
- Long noncoding RNA study: Genome-wide approaches.Genes & diseases · 2023Review
- Heterogeneous expression of alternatively spliced lncRNA mediates vascular smooth cell plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Elastin, arterial mechanics, and stenosis.American journal of physiology. Cell physiology · 2022Review
- Effect of Extracellular Vesicles From Multiple Cells on Vascular Smooth Muscle Cells in Atherosclerosis.Frontiers in pharmacology · 2022Review
- Vascular smooth muscle cells in atherosclerosis: time for a re-assessment.Cardiovascular research · 2021Review
- Article
- Review
- An Integrative Study of Aortic mRNA/miRNA Longitudinal Changes in Long-Term LVAD Support.International journal of molecular sciences · 2021Article
- lncRNA-PACER upregulatesGastroenterology report · 2021Article
- Tools, techniques, and future opportunities for characterizing the mechanobiology of uterine myometrium.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Mechanosensitive smooth muscle cell phenotypic plasticity emerging from a null state and the balance between Rac and Rho.Cell reports · 2021Article
- Novel Transcript Discovery Expands the Repertoire of Pathologically-Associated, Long Non-Coding RNAs in Vascular Smooth Muscle Cells.International journal of molecular sciences · 2021Article
- Getting the big picture of cell-matrix interactions: High-throughput biomaterial platforms and systems-level measurements.Current opinion in solid state & materials science · 2020Article
- Atherosclerosis in Different Vascular Locations Unbiasedly Approached with Mouse Genetics.Genes · 2020Review
- Analysis of splice variants of the human protein disulfide isomerase (P4HB) gene.BMC genomics · 2020Article
- Integrating Biophysics in Toxicology.Cells · 2020Review
- Control of matrix stiffness promotes endodermal lineage specification by regulating SMAD2/3 via lncRNA LINC00458.Science advances · 2020Article
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Authors and funding
4 authors.
Funding
Abstract
objectiveVascular extracellular matrix stiffening is a risk factor for aortic and coronary artery disease. How matrix stiffening regulates the transcriptome profile of human aortic and coronary vascular smooth muscle cells (VSMCs) is not well understood. Furthermore, the role of long noncoding RNAs (lncRNAs) in the cellular response to stiffening has never been explored. This study characterizes the stiffness-sensitive (SS) transcriptome of human aortic and coronary VSMCs and identifies potential key lncRNA regulators of stiffness-dependent VSMC functions. APPROACH AND
resultsAortic and coronary VSMCs were cultured on hydrogel substrates mimicking physiological and pathological extracellular matrix stiffness. Total RNAseq was performed to compare the SS transcriptome profiles of aortic and coronary VSMCs. We identified 3098 genes (2842 protein coding and 157 lncRNA) that were stiffness sensitive in both aortic and coronary VSMCs (false discovery rate <1%). Hierarchical clustering revealed that aortic and coronary VSMCs grouped by stiffness rather than cell origin. Conservation analyses also revealed that SS genes were more conserved than stiffness-insensitive genes. These VSMC SS genes were less tissue-type specific and expressed in more tissues than stiffness-insensitive genes. Using unbiased systems analyses, we identified MALAT1 as an SS lncRNA that regulates stiffness-dependent VSMC proliferation and migration in vitro and in vivo.
conclusionsThis study provides the transcriptomic landscape of human aortic and coronary VSMCs in response to extracellular matrix stiffness and identifies novel SS human lncRNAs. Our data suggest that the SS transcriptome is evolutionarily important to VSMCs function and that SS lncRNAs can act as regulators of stiffness-dependent phenotypes.
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