ArticleJournal of neuroscience methods2018
Comparative one- and two-photon uncaging of MNI-glutamate and MNI-kainate on hippocampal CA1 neurons.
Article in Journal of neuroscience methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- A Metal-Organic Framework Platform Enabling Visible-to-Ultraviolet Upconversion-Driven Heterogeneous Photochemistry.JACS Au · 2026Article
- The Quinoline Photoremovable Group (PPG) Platform-A Medicinal Chemist's Approach for Photocage Development and Applications.Medicinal research reviews · 2025Review
- C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules.IUCrJ · 2024Article
- Reverse Engineering Caged Compounds: Design Principles for their Application in Biology.Angewandte Chemie (International ed. in English) · 2023Review
- Differential Subcellular Distribution and Release Dynamics of Cotransmitted Cholinergic and GABAergic Synaptic Inputs Modify Dopaminergic Neuronal Excitability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Insulin-like growth factor 1 regulates excitatory synaptic transmission in pyramidal neurons from adult prefrontal cortex.Neuropharmacology · 2022Article
- Optofluidic control of rodent learning using cloaked caged glutamate.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation.Methods in enzymology · 2019Article
- Optical probing of acetylcholine receptors on neurons in the medial habenula with a novel caged nicotine drug analogue.The Journal of physiology · 2018Article
- Thermodynamically Stable, Photoreversible Pharmacology in Neurons with One- and Two-Photon Excitation.Angewandte Chemie (International ed. in English) · 2018Article
- Two-Photon Uncaging of Glutamate.Frontiers in synaptic neuroscience · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe light-induced release of neurotransmitters from caging chromophores provides a powerful means to study the underlying receptors in a physiologically relevant context. Surprisingly, most caged neurotransmitters, including the widely used 4-methoxy-7-nitroindolinyl (MNI)-glutamate, show strong antagonism against GABA-A receptors. Kainate has been shown to exhibit a higher efficacy at glutamate receptors compared to glutamate itself. Thus, uncaging of kainate might allow the application of the caged compound at lower, less antagonistic concentrations. NEW
methodsThis study provides a detailed comparison of MNI-glutamate and MNI-kainate uncaging by different modes of one- and two-photon irradiation on hippocampal CA1 pyramidal neurons in acute brain slices. RESULTS/COMPARISON WITH EXISTING
methodsUnexpectedly, the data revealed that currents in response to MNI-glutamate uncaging were larger compared to MNI-kainate with local one-photon laser uncaging at the soma and two-photon uncaging at the same spines. Furthermore, the direct comparison demonstrates the influence of type of caged agonist and light delivery conditions used for uncaging on the amplitude and kinetic properties of the current response.
conclusionThese findings highlight the importance of experimental design for uncaging experiments and provide a basis for future studies employing one- and two-photon uncaging to understand glutamate-dependent processes. It further provides the first example of two-photon uncaging of kainate at single spines in acute brain slices.
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