ArticleMethods (San Diego, Calif.)2018
Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells - Tools for analyzing dynamics of cell cycle, migration, and lineage selection.
Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- In Vitro Anti-Breast Cancer Effects ofInternational journal of molecular sciences · 2026Article
- CD44 Knockout Alters the Cellular Response and Improves Locomotor Outcome After Spinal Cord Injury.ASN neuro · 2026Article
- Cell Population Dynamics Informed by Cell-Cycle Regulation: A Deterministic Modeling Toolkit.Computational and structural biotechnology journal · 2026Review
- C1q drives neural stem cell quiescence by regulating cell cycle and metabolism through BAI1.Nature communications · 2025Article
- Enhanced Unidirectional Cell Migration Induced by Asymmetrical Micropatterns with Nanostructures.Journal of functional biomaterials · 2025Article
- LivecellX: A Scalable Deep Learning Framework for Single-Cell Object-Oriented Analysis in Live-Cell Imaging.bioRxiv : the preprint server for biology · 2025Article
- Time-Lapse Imaging to Analyze Cell Fate in Response to Antimitotics.Methods in molecular biology (Clifton, N.J.) · 2025Article
- MetaPhenotype: A Transferable Meta-Learning Model for Single-Cell Mass Spectrometry-Based Cell Phenotype Prediction Using Limited Number of Cells.Analytical chemistry · 2024Article
- Impact of Protein Coronas on Lipid Nanoparticle Uptake and Endocytic Pathways in Cells.Molecules (Basel, Switzerland) · 2024Article
- Towards 'end-to-end' analysis and understanding of biological timecourse data.The Biochemical journal · 2022Article
- Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments.PloS one · 2022Article
- Continuous Real-Time Motility Analysis ofPathogens (Basel, Switzerland) · 2021Article
- Engineering (Bio)Materials through Shrinkage and Expansion.Advanced healthcare materials · 2021Review
- Best Practices and Progress in Precision-Cut Liver Slice Cultures.International journal of molecular sciences · 2021Review
- Computational Methods for Single-Cell Imaging and Omics Data Integration.Frontiers in molecular biosciences · 2021Review
- Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair.eLife · 2020Article
- Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling.ACS chemical biology · 2020Article
- Tools and Concepts for Interrogating and Defining Cellular Identity.Cell stem cell · 2020Review
- When blood development meets single-cell transcriptomics.Blood science (Baltimore, Md.) · 2019Article
- Uncoupling Traditional Functionalities of Metastasis: The Parting of Ways with Real-Time Assays.Journal of clinical medicine · 2019Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Neural stem cell (NSC) cultures have been considered technically challenging for time-lapse analysis due to high motility, photosensitivity, and growth at confluent densities. We have tested feasibility of long-term live-cell time-lapse analysis for NSC migration and differentiation studies. Here, we describe a method to study the dynamics of cell cycle, migration, and lineage selection in cultured multipotent mouse or human NSCs using single-cell tracking during a long-term, 7-14 day live-cell time-lapse analysis. We used in-house made PDMS inserts with five microwells on a glass coverslip petri-dish to constrain NSC into the area of acquisition during long-term live-cell imaging. In parallel, we have defined image acquisition settings for single-cell tracking of cell cycle dynamics using Fucci-reporter mouse NSC for 7 days as well as lineage selection and migration using human NSC for 14 days. Overall, we show that adjustments of live-cell analysis settings can extend the time period of single-cell tracking in mouse or human NSC from 24-72 h up to 7-14 days and potentially longer. However, we emphasize that experimental use of repeated fluorescence imaging will require careful consideration of controls during acquisition and analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.