ArticleParasites & vectors2017
Isothermal diagnostic assays for the detection of soil-transmitted helminths based on the SmartAmp2 method.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol.PLoS neglected tropical diseases · 2018Trial
- Hunting for Helminths: short- and long-read shotgun metagenomics for helminth detection in faecal samples.PLoS neglected tropical diseases · 2026Article
- Molecular characterization of the prevalent soil-transmitted helminths in Narathiwat Province, southern Thailand.PloS one · 2026Article
- NTDscope: A multi-contrast portable microscope for disease diagnosis.PLOS global public health · 2026Article
- Development of loop mediated isothermal amplification assay for rapid detection and its comparison with PCR techniques for Ancylostoma duodenale.Scientific reports · 2025Article
- Use of a Novel Real-Time PCR to Investigate Anthelmintic Efficacy AgainstVeterinary sciences · 2025Article
- Advantages and Limitations of Microscopy and Molecular Detections for Diagnosis of Soil-transmitted Helminths: An Overview.Helminthologia · 2022Article
- Worms and bugs of the gut: the search for diagnostic signatures using barcoding, and metagenomics-metabolomics.Parasites & vectors · 2022Review
- Sensitive and semiquantitative detection of soil-transmitted helminth infection in stool using a recombinase polymerase amplification-based assay.PLoS neglected tropical diseases · 2021Article
- Diagnostic Techniques for Soil-Transmitted Helminths - Recent Advances.Research and reports in tropical medicine · 2021Review
- Challenges and opportunities for the adoption of molecular diagnostics for anthelmintic resistance.International journal for parasitology. Drugs and drug resistance · 2020Article
- Whip-LAMP: a novel LAMP assay for the detection of Trichuris muris-derived DNA in stool and urine samples in a murine experimental infection model.Parasites & vectors · 2020Article
- Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.International journal of molecular sciences · 2020Review
- Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).Virology journal · 2020Article
- Diagnostic Techniques of Soil-Transmitted Helminths: Impact on Control Measures.Tropical medicine and infectious disease · 2020Review
- Whipworm and roundworm infections.Nature reviews. Disease primers · 2020Review
- Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development.Parasitology · 2020Review
- Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.Infectious diseases of poverty · 2019Review
- Toward the 2020 goal of soil-transmitted helminthiasis control and elimination.PLoS neglected tropical diseases · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDiagnosis of soil-transmitted helminths (STHs) has traditionally relied on stool microscopy, which has a number of critical deficiencies. Molecular diagnostics are powerful tools to identify closely related species, but the requirement for costly equipment makes their implementation difficult in low-resource or field settings. Rapid, sensitive and cost-effective diagnostic tools are crucial for accurate estimation of STH infection intensity in MDA programmes in which the goal is to reduce morbidity following repeated rounds of chemotherapy.
resultsIn this study, colourimetric isothermal assays were developed using SmartAmp2 primer sets and reagents in loop-mediated amplification (LAMP) assays. Species-specific primer sets, designed on a specific target sequence in the β-tubulin gene, were used to identify Necator americanus, Trichuris trichiura and Ascaris lumbricoides. After initial optimization on control plasmids and genomic DNA from adult worms, assays were evaluated on field samples. Assays showed high sensitivity and demonstrated high tolerance to inhibitors in spiked faecal samples. Rapid and sensitive colourimetric assays were successfully developed to identify the STHs in field samples using hydroxy napthol blue (HNB) dye.
conclusionsRapid and simple colourimetric diagnostic assays, using the SmartAmp2 method, were developed, with the potential to be applied in the field for detection of STH infections and the estimation of response to treatment. However, further validation on large numbers of field samples is needed.
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