Evidence map›Paper›PMID 29046708›Full record

ArticlePlant methods2017

Improved method for genomic DNA extraction for

César Ramiro Martínez-González, Rosario Ramírez-Mendoza, Jaime Jiménez-Ramírez, Clemente Gallegos-Vázquez, Isolda Luna-Vega

Abstract read
In one paragraph

Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
  2. Potential ofPlants (Basel, Switzerland) · 2025
    Article
  3. New Species ofJournal of fungi (Basel, Switzerland) · 2025
    Article
  4. Article
  5. Article
  6. Eleven New Species of the GenusJournal of fungi (Basel, Switzerland) · 2024
    Article
  7. Whole-genome sequence ofMicrobiology resource announcements · 2023
    Article
  8. Article
  9. Five New Species ofJournal of fungi (Basel, Switzerland) · 2023
    Article
  10. Article
  11. New Ascomycetes from the Mexican Tropical Montane Cloud Forest.Journal of fungi (Basel, Switzerland) · 2023
    Article
  12. Article
  13. MycoKeys · 2023
    Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

César Ramiro Martínez-GonzálezLaboratorio de Biogeografía y Sistemática, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510 Mexico City, México.
Rosario Ramírez-MendozaLaboratorio de Biotecnología de Semillas, Colegio de Postgraduados, Carretera México-Texcoco, 56230 Estado de México, México.
Jaime Jiménez-RamírezHerbario de la Facultad de Ciencias, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510 Mexico City, México.
Clemente Gallegos-VázquezCentro Regional Universitario Centro Norte, Universidad Autónoma Chapingo, Cruz del Sur núm. 100, Colonia Constelación, El Orito, 98085 Zacatecas, Zacatecas México.
Isolda Luna-VegaLaboratorio de Biogeografía y Sistemática, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510 Mexico City, México.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGenomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin is one of the main components of cellular walls, whereas mucilage is a complex polysaccharide with a ramified structure. Thus, pectin- and mucilage-free extraction of DNA is a key step for further downstream PCR-based analyses.

resultsWe tested our DNA extraction method on cladode tissue (juvenile, adult, and herbaria exemplars) of 17 species of

conclusionWe developed a method for the extraction of gDNA free of inhibitory compounds common in species of

Indexed as

DNA qualityDNA quantityGenomic DNAMucilageOpuntiaPectin

Identifiers

PMID29046708
PMCPMC5637266

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.