ArticleFrontiers in plant science2017
An Updated Protocol for High Throughput Plant Tissue Sectioning.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- The genetic architecture of leaf vein density and its importance for photosynthesis in maize.The New phytologist · 2026Article
- The Rapid Anatomics Tool (RAT): A low-cost root anatomical phenotyping platform reveals changes in root anatomy along the root axis.Plant phenomics (Washington, D.C.) · 2026Article
- Pangenome-Wide Identification, Evolutionary Analysis of Maize ZmPLD Gene Family, and Functional Validation of ZmPLD15 in Cold Stress Tolerance.Plants (Basel, Switzerland) · 2025Article
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- Use of polyethylene glycol as an alternative to optimal cutting temperature medium in freeze sectioning for plant histochemical studies.Protoplasma · 2025Article
- Standardization of pharmacognostic characters and phytochemical study of Limeum obovatum vicary.Scientific reports · 2025Article
- Preparation and Sectioning of Paraffin-Embedded Tissue for Histology and Histochemistry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Constitutive basis of root system architecture: uncovering a promising trait for breeding nutrient- and drought-resilient crops.aBIOTECH · 2023Article
- Natural variation of maize root hydraulic architecture underlies highly diverse water uptake capacities.Plant physiology · 2023Article
- Trans-species microRNA loci in the parasitic plant Cuscuta campestris have a U6-like snRNA promoter.The Plant cell · 2023Article
- The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning.Plant methods · 2023Article
- Simple, Fast and Efficient Methods for Analysing the Structural, Ultrastructural and Cellular Components of the Cell Wall.Plants (Basel, Switzerland) · 2022Article
- Soil penetration by maize roots is negatively related to ethylene-induced thickening.Plant, cell & environment · 2022Article
- Non-invasive Imaging of Rice Roots in Non-compacted and Compacted Soil.Bio-protocol · 2021Article
- Deep soil exploration vs. topsoil exploitation: distinctive rooting strategies between wheat landraces and wild relatives.Plant and soil · 2021Article
- The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice.Development (Cambridge, England) · 2020Article
- Methanol fixation for scanning electron microscopy of plants.Applied microscopy · 2020Review
- GRANAR, a Computational Tool to Better Understand the Functional Importance of Monocotyledon Root Anatomy.Plant physiology · 2020Article
- A 3D Print Repository for Plant Phenomics.Plant phenomics (Washington, D.C.) · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily of root tissue but which can also be readily applied to other tissues such as leaves or stems. To increase the throughput of traditional agarose embedding and sectioning, custom designed 3D printed molds were utilized to embed 5-15 roots in a block for sectioning in a single cut. A single fluorescent stain in combination with laser scanning confocal microscopy was used to obtain high quality images of thick sections. The provided CAD files allow production of the embedding molds described here from a number of online 3D printing services. Although originally developed for roots, this method provides rapid, high quality cross sections of many plant tissue types, making it suitable for use in forward genetic screens for differences in specific cell structures or developmental changes. To demonstrate the utility of the technique, the two parent lines of the wheat (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.