ArticleMetabolomics : Official journal of the Metabolomic Society2017
The dental calculus metabolome in modern and historic samples.
Article in Metabolomics : Official journal of the Metabolomic Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 64 citations in OpenAlex.
- The Oral Microbiome of King Richard III of England.American journal of biological anthropology · 2026Article
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- Mass Spectrometry-Based Oral Meta-Omics Analysis-What We Know and What We Don't.Advances in experimental medicine and biology · 2026Review
- Unearthing prehistoric diets: First evidence of horse meat consumption in Early Bronze Age Sicily.PloS one · 2025Article
- Paleomicrobiology: Tracking the past microbial life from single species to entire microbial communities.Microbial biotechnology · 2024Review
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- Curated cauldrons: Preserved proteins from early copper-alloy vessels illuminate feasting practices in the Caucasian steppe.iScience · 2023Article
- Metabolomics in archaeological science: A review of their advances and present requirements.Science advances · 2023Review
- Ultra-high performance liquid chromatography high-resolution mass spectrometry for metabolomic analysis of dental calculus from Duke Alessandro Farnese and Maria D'Aviz.Scientific reports · 2023Article
- Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case ofMetabolites · 2023Article
- Assessment and Correlation of Salivary Ca, Mg, and pH in Smokers and Non-Smokers with Generalized Chronic Periodontitis.Medicina (Kaunas, Lithuania) · 2023Article
- Paleoproteomic evidence reveals dairying supported prehistoric occupation of the highland Tibetan Plateau.Science advances · 2023Article
- Examination of human osteoarchaeological remains as a feasible source of polar and apolar metabolites to study past conditions.Scientific reports · 2023Article
- Ancient dental calculus preserves signatures of biofilm succession and interindividual variation independent of dental pathology.PNAS nexus · 2022Article
- Comparison of oral metabolome profiles of stimulated saliva, unstimulated saliva, and mouth-rinsed water.Scientific reports · 2022Article
- Dental calculus: A repository of bioinformation indicating diseases and human evolution.Frontiers in cellular and infection microbiology · 2022Review
- Metagenomic analysis of ancient dental calculus reveals unexplored diversity of oral archaeal Methanobrevibacter.Microbiome · 2021Article
- Characteristics of Dietary Fatty Acids Isolated from Historic Dental Calculus of the 17th- and 18th-Century Inhabitants of the Subcarpathian Region (Poland).Molecules (Basel, Switzerland) · 2021Article
- Article
- Multi-omic detection ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
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Authors and funding
14 authors at 10 institutions in 4 countries.
Funding
Abstract
introductionDental calculus is a mineralized microbial dental plaque biofilm that forms throughout life by precipitation of salivary calcium salts. Successive cycles of dental plaque growth and calcification make it an unusually well-preserved, long-term record of host-microbial interaction in the archaeological record. Recent studies have confirmed the survival of authentic ancient DNA and proteins within historic and prehistoric dental calculus, making it a promising substrate for investigating oral microbiome evolution via direct measurement and comparison of modern and ancient specimens.
objectiveWe present the first comprehensive characterization of the human dental calculus metabolome using a multi-platform approach.
methodsUltra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) quantified 285 metabolites in modern and historic (200 years old) dental calculus, including metabolites of drug and dietary origin. A subset of historic samples was additionally analyzed by high-resolution gas chromatography-MS (GC-MS) and UPLC-MS/MS for further characterization of metabolites and lipids. Metabolite profiles of modern and historic calculus were compared to identify patterns of persistence and loss.
resultsDipeptides, free amino acids, free nucleotides, and carbohydrates substantially decrease in abundance and ubiquity in archaeological samples, with some exceptions. Lipids generally persist, and saturated and mono-unsaturated medium and long chain fatty acids appear to be well-preserved, while metabolic derivatives related to oxidation and chemical degradation are found at higher levels in archaeological dental calculus than fresh samples.
conclusionsThe results of this study indicate that certain metabolite classes have higher potential for recovery over long time scales and may serve as appropriate targets for oral microbiome evolutionary studies.
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