ArticleBiochemistry2017
Metal Dependence of the Xylose Isomerase from Piromyces sp. E2 Explored by Activity Profiling and Protein Crystallography.
Article in Biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production.Biotechnology journal · 2025Article
- Big data mining, rational modification, and ancestral sequence reconstruction inferred multiple xylose isomerases for biorefinery.Science advances · 2023Article
- A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion.Biotechnology for biofuels and bioproducts · 2022Article
- Nucleation of glucose isomerase protein crystals in a nonclassical disguise: The role of crystalline precursors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Exploring metal ion metabolisms to improve xylose fermentation in Saccharomyces cerevisiae.Microbial biotechnology · 2021Article
- Pentose degradation in archaea: Halorhabdus species degrade D-xylose, L-arabinose and D-ribose via bacterial-type pathways.Extremophiles : life under extreme conditions · 2020Article
- Structure-based directed evolution improvesBiotechnology for biofuels · 2020Article
- Structural knowledge or X-ray damage? A case study on xylose isomerase illustrating both.Journal of synchrotron radiation · 2019Article
- Reassessment of requirements for anaerobic xylose fermentation by engineered, non-evolved Saccharomyces cerevisiae strains.FEMS yeast research · 2019Article
- A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation.Frontiers in microbiology · 2019Article
- Engineering of Pentose Transport inFrontiers in bioengineering and biotechnology · 2019Review
- Structural insight intoScientific reports · 2018Article
- Genomic and phenotypic characterization of a refactored xylose-utilizingBiotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylose isomerase from Piromyces sp. E2 (PirXI) can be used to equip Saccharomyces cerevisiae with the capacity to ferment xylose to ethanol. The biochemical properties and structure of the enzyme have not been described even though its metal content, catalytic parameters, and expression level are critical for rapid xylose utilization. We have isolated the enzyme after high-level expression in Escherichia coli, analyzed the metal dependence of its catalytic properties, and determined 12 crystal structures in the presence of different metals, substrates, and substrate analogues. The activity assays revealed that various bivalent metals can activate PirXI for xylose isomerization. Among these metals, Mn
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