ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2018
Diagnostics for Yaws Eradication: Insights From Direct Next-Generation Sequencing of Cutaneous Strains of Treponema pallidum.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Whole-genome sequencing reveals evidence for inter-species transmission of the yaws bacterium among nonhuman primates in Tanzania.PLoS neglected tropical diseases · 2025Article
- Yaws in Africa: Past, Present and Future.Diseases (Basel, Switzerland) · 2025Review
- Insights into Treponema pallidum genomics from modern and ancient genomes using a novel mapping strategy.BMC biology · 2025Article
- Article
- Low genetic diversity of Treponema pallidum ssp. pertenue (TPE) isolated from patients' ulcers in Namatanai District of Papua New Guinea: Local human population is infected by three TPE genotypes.PLoS neglected tropical diseases · 2024Article
- The genomes of the yaws bacterium, Treponema pallidum subsp. pertenue, of nonhuman primate and human origin are not genomically distinct.PLoS neglected tropical diseases · 2023Article
- Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing.BMC infectious diseases · 2023Article
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Treponema pallidum SubspeciesMicrobiology spectrum · 2022Article
- Yaws in the Philippines: A clinico-seroprevalence study of selected communities in Mindanao.PLoS neglected tropical diseases · 2022Article
- LAMP4yaws:BMJ open · 2022Article
- Evolutionary Processes in the Emergence and Recent Spread of the Syphilis Agent, Treponema pallidum.Molecular biology and evolution · 2022Article
- Global phylogeny of Treponema pallidum lineages reveals recent expansion and spread of contemporary syphilis.Nature microbiology · 2021Article
- Epidemiologic and Genomic Reidentification of Yaws, Liberia.Emerging infectious diseases · 2021Article
- A Loop-Mediated Isothermal Amplification Assay for the Detection ofThe American journal of tropical medicine and hygiene · 2020Article
- Targeted surveillance strategies for efficient detection of novel antibiotic resistance variants.eLife · 2020Article
- Genomic epidemiology of syphilis reveals independent emergence of macrolide resistance across multiple circulating lineages.Nature communications · 2019Article
- Identification of positively selected genes in human pathogenic treponemes: Syphilis-, yaws-, and bejel-causing strains differ in sets of genes showing adaptive evolution.PLoS neglected tropical diseases · 2019Article
- Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens.Frontiers in microbiology · 2019Article
- Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Indonesia: Modular structure of several treponemal genes.PLoS neglected tropical diseases · 2018Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
Background: Yaws-like chronic ulcers can be caused by Treponema pallidum subspecies pertenue, Haemophilus ducreyi, or other, still-undefined bacteria. To permit accurate evaluation of yaws elimination efforts, programmatic use of molecular diagnostics is required. The accuracy and sensitivity of current tools remain unclear because our understanding of T. pallidum diversity is limited by the low number of sequenced genomes. Methods: We tested samples from patients with suspected yaws collected in the Solomon Islands and Ghana. All samples were from patients whose lesions had previously tested negative using the Centers for Disease Control and Prevention (CDC) diagnostic assay in widespread use. However, some of these patients had positive serological assays for yaws on blood. We used direct whole-genome sequencing to identify T. pallidum subsp pertenue strains missed by the current assay. Results: From 45 Solomon Islands and 27 Ghanaian samples, 11 were positive for T. pallidum DNA using the species-wide quantitative polymerase chain reaction (PCR) assay, from which we obtained 6 previously undetected T. pallidum subsp pertenue whole-genome sequences. These show that Solomon Islands sequences represent distinct T. pallidum subsp pertenue clades. These isolates were invisible to the CDC diagnostic PCR assay, due to sequence variation in the primer binding site. Conclusions: Our data double the number of published T. pallidum subsp pertenue genomes. We show that Solomon Islands strains are undetectable by the PCR used in many studies and by health ministries. This assay is therefore not adequate for the eradication program. Next-generation genome sequence data are essential for these efforts.
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