ArticlePloS one2017
The ethylene response factor AtERF4 negatively regulates the iron deficiency response in Arabidopsis thaliana.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 62 citations in OpenAlex.
- Bacillus velezensis 83 protects Arabidopsis thaliana against Botrytis cinerea by triggering JA-, and SA-dependent induced systemic resistance.Pest management science · 2026Article
- Transcriptomics Data Mining to Identify Novel Regulatory Genes of Iron Uptake in Drought-Stressed Wheat.International journal of molecular sciences · 2025Article
- Variability in Leaf Color Induced by Chlorophyll Deficiency: Transcriptional Changes in Bamboo Leaves.Current issues in molecular biology · 2024Article
- Transcriptome-based network analysis of cell cycle-related genes in response to blue and red light in maize.AoB PLANTS · 2023Article
- Research progress on iron absorption, transport, and molecular regulation strategy in plants.Frontiers in plant science · 2023Review
- Advances in Iron Retrograde Signaling Mechanisms and Uptake Regulation in Photosynthetic Organisms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Transcriptomic comparison sheds new light on regulatory networks for dimorphic flower development in response to photoperiod in Viola prionantha.BMC plant biology · 2022Article
- Integrated Transcriptomics and Nontargeted Metabolomics Analysis Reveal Key Metabolic Pathways inJournal of fungi (Basel, Switzerland) · 2022Article
- Ethylene Response Factor109 Attunes Immunity, Photosynthesis, and Iron Homeostasis in Arabidopsis Leaves.Frontiers in plant science · 2022Article
- Interdependent iron and phosphorus availability controls photosynthesis through retrograde signaling.Nature communications · 2021Article
- Gadolinium ProtectsInternational journal of molecular sciences · 2021Article
- Ethylene and Nitric Oxide Involvement in the Regulation of Fe and P Deficiency Responses in Dicotyledonous Plants.International journal of molecular sciences · 2021Review
- Genetic regulators of leaf size in Brassica crops.Horticulture research · 2021Review
- ERF4 and MYB52 transcription factors play antagonistic roles in regulating homogalacturonan de-methylesterification in Arabidopsis seed coat mucilage.The Plant cell · 2021Article
- Article
- Genome-Wide Analysis and the Expression Pattern of the ERF Gene Family inPlants (Basel, Switzerland) · 2021Article
- Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings.BMC plant biology · 2020Article
- Ethylene-responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon.Plant biotechnology journal · 2020Article
- The Transcriptional Control of Iron Homeostasis in Plants: A Tale of bHLH Transcription Factors?Frontiers in plant science · 2019Article
- Induced Systemic Resistance (ISR) and Fe Deficiency Responses in Dicot Plants.Frontiers in plant science · 2019Review
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Authors and funding
8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Iron (Fe) deficiency is one of many conditions that can seriously damage crops. Low levels of photosynthesis can lead to the degradation of chlorophyll content and impaired respiration in affected plants, which together cause poor growth and reduce quality. Although ethylene plays an important role in responses to Fe deficiency, a limited number of studies have been carried out on ethylene response factor (ERFs) as components of plant regulation mechanisms. Thus, this study aimed to investigate the role of AtERF4 in plant responses to Fe deficiency. Results collected when Arabidopsis thaliana was grown under Fe deficient conditions as well as in the presence of 1-aminocyclopropane-1-carboxylic acid (ACC) revealed that leaf chlorosis did not occur over short timescales and that chloroplast structural integrity was retained. At the same time, expression of the chlorophyll degradation-related genes AtPAO and AtCLH1 was inhibited and net H+ root flux was amplified. Our results show that chlorophyll content was enhanced in the mutant erf4, while expression of the chlorophyll degradation gene AtCLH1 was reduced. Ferric reductase activity in roots was also significantly higher in the mutant than in wild type plants, while erf4 caused high levels of expression of the genes AtIRT1 and AtHA2 under Fe deficient conditions. We also utilized yeast one-hybrid technology in this study to determine that AtERF4 binds directly to the AtCLH1 and AtITR1 promoter. Observations show that transient over-expression of AtERF4 resulted in rapid chlorophyll degradation in the leaves of Nicotiana tabacum and the up-regulation of gene AtCLH1 expression. In summary, AtERF4 plays an important role as a negative regulator of Fe deficiency responses, we hypothesize that AtERF4 may exert a balancing effect on plants subject to nutrition stress.
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