ArticlePlant biotechnology journal2018
Overexpression of serine acetyltransferase in maize leaves increases seed-specific methionine-rich zeins.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Unveiling the Link Between Expression of Aspartate Kinase2 (ask2) Gene and Accumulation of Methionine in Developing Maize Kernels.Biochemical genetics · 2026Article
- Allelic Diversity and Development of Breeder-Friendly Marker Specific to floury2 Gene Regulating the Accumulation of α-Zeins and Essential Amino Acids in Maize Kernel.Biochemical genetics · 2025Article
- Identification and Expression Analysis of the Soybean Serine Acetyltransferase (International journal of molecular sciences · 2025Article
- High-quality bonds:Frontiers in plant science · 2025Article
- Catalysts for sulfur: understanding the intricacies of enzymes orchestrating plant sulfur anabolism.Planta · 2024Review
- Temporal profile of amino acids and protein fractions in the developing kernel of maize germplasm.Scientific reports · 2024Article
- Molecular characterization, haplotype analysis and development of markers specific to3 Biotech · 2024Article
- The Serine Acetyltransferase (International journal of molecular sciences · 2024Article
- Genotyping-by-Sequencing Analysis Reveals Associations between Agronomic and Oil Traits in Gamma Ray-Derived Mutant Rapeseed (Plants (Basel, Switzerland) · 2024Article
- Assessing sequence variation, haplotype analysis and molecular characterisation of aspartate kinase2 (ask2) gene regulating methionine biosynthesis in diverse maize inbreds.Molecular genetics and genomics : MGG · 2024Article
- Sulfate transport and metabolism: strategies to improve the seed protein quality.Molecular biology reports · 2024Review
- Improved pearl millet genomes representing the global heterotic pool offer a framework for molecular breeding applications.Communications biology · 2023Article
- Genetic and molecular understanding for the development of methionine-rich maize: a holistic approach.Frontiers in plant science · 2023Review
- Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato.Frontiers in plant science · 2022Article
- Co-overexpression ofFrontiers in plant science · 2022Article
- Genetic basis of maize kernel protein content revealed by high-density bin mapping using recombinant inbred lines.Frontiers in plant science · 2022Article
- Genome-wide identification of serine acetyltransferase (SAT) gene family in rice (Oryza sativa) and their expressions under salt stress.Molecular biology reports · 2021Article
- Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata.Plant biotechnology journal · 2021Article
- Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize.Genes · 2021Article
- Overexpression of ATP sulfurylase improves the sulfur amino acid content, enhances the accumulation of Bowman-Birk protease inhibitor and suppresses the accumulation of the β-subunit of β-conglycinin in soybean seeds.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize kernels do not contain enough of the essential sulphur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulphur (S)-amino acids may limit their incorporation into seed storage proteins. Serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis, and SAT overexpression is known to enhance S-assimilation without negative impact on plant growth. Therefore, we overexpressed Arabidopsis thaliana AtSAT1 in maize under control of the leaf bundle sheath cell-specific rbcS1 promoter to determine the impact on seed storage protein expression. The transgenic events exhibited up to 12-fold higher SAT activity without negative impact on growth. S-assimilation was increased in the leaves of SAT overexpressing plants, followed by higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa δ-zein, during endosperm development. This zein is known to impact the level of Met stored in kernels. The elite event with the highest expression of AtSAT1 showed 1.40-fold increase in kernel Met. When fed to chickens, transgenic AtSAT1 kernels significantly increased growth rate compared with the parent maize line. The result demonstrates the efficacy of increasing maize nutritional value by SAT overexpression without apparent yield loss. Maternal overexpression of SAT in vegetative tissues was necessary for high-Met zein accumulation. Moreover, SAT overcomes the shortage of S-amino acids that limits the expression and accumulation of high-Met zeins during kernel development.
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