ArticleAngewandte Chemie (International ed. in English)2017
Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers.
Article in Angewandte Chemie (International ed. in English), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- ICE-CAP: ice crystal engineering for cryogel architecture programming. Modulation of material properties with ice-binding polymers.Materials horizons · 2026Article
- Core-block engineering enables control of ice recrystallisation inhibition in polymer nanoparticles.Chemical science · 2026Article
- Antifreezing hydrogels for biomedical applications from design strategies to emerging multifunctionality.Bioactive materials · 2026Review
- An Ultrapotent, Ultraeconomical, Antifreeze Polypeptide.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Adsorption Barrier Limits the Ice Inhibition Activity of Glycan-Rich Antifreeze Glycoproteins.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- The mechanism and protective strategies of follicle injury after ovarian tissue cryopreservation and thawed transplantation: a review.Journal of ovarian research · 2025Review
- Atomistic Insights into the Chain-Length-Dependent Antifreeze Activity of Oligoprolines.Biomacromolecules · 2025Article
- Recent Advances in Antifreeze Peptide Preparation: A Review.Molecules (Basel, Switzerland) · 2024Review
- Synthetic Antifreeze Glycoproteins with Potent Ice-Binding Activity.Chemistry of materials : a publication of the American Chemical Society · 2024Article
- Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities.Biomaterials science · 2023Article
- Post-thaw application of ROCK-inhibitors increases cryopreserved T-cell yield.RSC medicinal chemistry · 2023Article
- High Molecular Weight Polyproline as a Potential Biosourced Ice Growth Inhibitor: Synthesis, Ice Recrystallization Inhibition, and Specific Ice Face Binding.Biomacromolecules · 2023Article
- Article
- Empirical Optimization of Peptide Sequence and Nanoparticle Colloidal Stability: The Impact of Surface Ligands and Implications for Colorimetric Sensing.ACS applied materials & interfaces · 2023Article
- Microcurvature Controllable Metal-Organic Framework Nanoagents Capable of Ice-Lattice Matching for Cellular Cryopreservation.JACS Au · 2023Article
- Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants.ACS applied materials & interfaces · 2023Article
- Synthesis of Poly(2-(methylsulfinyl)ethyl methacrylate) via Oxidation of Poly(2-(methylthio)ethyl methacrylate): Evaluation of the Sulfoxide Side Chain on Cryopreservation.ACS polymers Au · 2022Article
- Spacer Matters: All-Peptide-Based Ligand for Promoting Interfacial Proteolysis and Plasmonic Coupling.Nano letters · 2022Article
- Memory Effect in Thermoresponsive Proline-based Polymers.Angewandte Chemie (International ed. in English) · 2022Article
- Bioinspired Threonine-Based Polymers with Potent Ice Recrystallization Inhibition Activity.ACS applied polymer materials · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Tissue engineering, gene therapy, drug screening, and emerging regenerative medicine therapies are fundamentally reliant on high-quality adherent cell culture, but current methods to cryopreserve cells in this format can give low cell yields and require large volumes of solvent "antifreezes". Herein, we report polyproline as a minimum (bio)synthetic mimic of antifreeze proteins that is accessible by solution, solid-phase, and recombinant methods. We demonstrate that polyproline has ice recrystallisation inhibition activity linked to its amphipathic helix and that it enhances the DMSO cryopreservation of adherent cell lines. Polyproline may be a versatile additive in the emerging field of macromolecular cryoprotectants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.