ArticleJournal of cellular physiology2018
Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells.
Article in Journal of cellular physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Mechanobiology of Matricellular Proteins in Bladder Cancer: A Narrative Review and Bioinformatics Analysis.Biomolecules · 2026Review
- Immune cells during compensatory renal hypertrophy after unilateral nephrectomy.Research square · 2026Article
- A microphysiologic human cervical model recapitulates microbial, immune, and pathogenic properties of sexually transmitted infections.Science advances · 2026Article
- Modular Parallel Plate Flow Chamber with Tunable Substrate Mechanics and Defined Shear Stress.bioRxiv : the preprint server for biology · 2025Article
- Cellular elasticity drives mechano-adaptation against fluid shear stress.Journal of cell science · 2025Article
- Shear stress effects on epididymal epithelial cell via primary cilia mechanosensory signaling.Journal of cellular physiology · 2025Article
- Advanced Kidney Models In Vitro Using the Established Cell Line Renal Proximal Tubular Epithelial/Telomerase Reverse Transcriptase1 for Nephrotoxicity Assays.Biomimetics (Basel, Switzerland) · 2024Article
- Decoupling shear stress and pressure effects in the biomechanics of autosomal dominant polycystic kidney disease using a perfused kidney-on-chip.bioRxiv : the preprint server for biology · 2024Article
- Cold-shock proteins accumulate in centrosomes and their expression and primary cilium morphology are regulated by hypothermia and shear stress.Histology and histopathology · 2024Article
- Status of breast cancer detection in young women and potential of liquid biopsy.Frontiers in oncology · 2024Review
- Development of a Highly Differentiated Human Primary Proximal Tubule MPS Model (aProximate MPS Flow).Bioengineering (Basel, Switzerland) · 2023Article
- Multi-Omics of Familial Thoracic Aortic Aneurysm and Dissection: Calcium Transport Impairment Predisposes Aortas to Dissection.International journal of molecular sciences · 2023Article
- Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study.Pharmacological reports : PR · 2023Article
- Research progress on the mechanism of renal interstitial fibrosis in obstructive nephropathy.Heliyon · 2023Review
- Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock.PLoS genetics · 2023Article
- Fluid shear stress triggers cholesterol biosynthesis and uptake in inner medullary collecting duct cells, independently of nephrocystin-1 and nephrocystin-4.Frontiers in molecular biosciences · 2023Article
- JAK/STAT pathway: Extracellular signals, diseases, immunity, and therapeutic regimens.Frontiers in bioengineering and biotechnology · 2023Review
- Evaluation of rapid transepithelial electrical resistance (TEER) measurement as a metric of kidney toxicity in a high-throughput microfluidic culture system.Scientific reports · 2022Article
- Novel 3D capsule device to restrict kidney volume expansion on polycystic kidney progression: feasibility study in a rat model.Journal of nephrology · 2022Article
- Innate Bacteriostatic Mechanisms Defend the Urinary Tract.Annual review of physiology · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Renal epithelial cells are exposed to mechanical forces due to flow-induced shear stress within the nephrons. Shear stress is altered in renal diseases caused by tubular dilation, obstruction, and hyperfiltration, which occur to compensate for lost nephrons. Fundamental in regulation of shear stress are primary cilia and other mechano-sensors, and defects in cilia formation and function have profound effects on development and physiology of kidneys and other organs. We applied RNA sequencing to get a comprehensive overview of fluid-shear regulated genes and pathways in renal epithelial cells. Functional enrichment-analysis revealed TGF-β, MAPK, and Wnt signaling as core signaling pathways up-regulated by shear. Inhibitors of TGF-β and MAPK/ERK signaling modulate a wide range of mechanosensitive genes, identifying these pathways as master regulators of shear-induced gene expression. However, the main down-regulated pathway, that is, JAK/STAT, is independent of TGF-β and MAPK/ERK. Other up-regulated cytokine pathways include FGF, HB-EGF, PDGF, and CXC. Cellular responses to shear are modified at several levels, indicated by altered expression of genes involved in cell-matrix, cytoskeleton, and glycocalyx remodeling, as well as glycolysis and cholesterol metabolism. Cilia ablation abolished shear induced expression of a subset of genes, but genes involved in TGF-β, MAPK, and Wnt signaling were hardly affected, suggesting that other mechano-sensors play a prominent role in the shear stress response of renal epithelial cells. Modulations in signaling due to variations in fluid shear stress are relevant for renal physiology and pathology, as suggested by elevated gene expression at pathological levels of shear stress compared to physiological shear.
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