Evidence map›Paper›PMID 29044197›Full record

ArticleScientific reports2017

Development of a multiplex probe combination-based one-step real-time reverse transcription-PCR for NA subtype typing of avian influenza virus.

Zhihao Sun, Tao Qin, Feifei Meng, Sujuan Chen, Daxin Peng, Xiufan Liu

Abstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhihao SunCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China.
Tao QinCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China.
Feifei MengCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China.
Sujuan ChenCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China.
Daxin PengCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China. pengdx@yzu.edu.cn.ORCID 0000-0001-6773-5149
Xiufan LiuCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nine influenza virus neuraminidase (NA) subtypes have been identified in poultry and wild birds. Few methods are available for rapid and simple NA subtyping. Here we developed a multiplex probe combination-based one-step real-time reverse transcriptase PCR (rRT-PCR) to detect nine avian influenza virus NA subtypes. Nine primer-probe pairs were assigned to three groups based on the different fluorescent dyes of the probes (FAM, HEX, or Texas Red). Each probe detected only one NA subtype, without cross reactivity. The detection limit was less than 100 EID

Indexed as

Multiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionAnimalsBirdsDNA PrimersInfluenza A virusInfluenza in BirdsNeuraminidasePoultrySensitivity and SpecificityViral ProteinsDNA PrimersNA protein, influenza A virusNeuraminidaseViral Proteins

Identifiers

PMID29044197
PMCPMC5647442

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.