ArticleScientific reports2017
Laboratory cryo x-ray microscopy for 3D cell imaging.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 71 citations in OpenAlex.
- Axial X-Ray Microscopy in Nanotomography.Tomography (Ann Arbor, Mich.) · 2026Article
- Sample Preparation Protocol for Laboratory Cryo-Soft X-Ray Microscopy for Studying Cellular Nanoparticle Uptake.International journal of molecular sciences · 2025Article
- Investigation of structural and reflective characteristics of short-period Mo/BJournal of synchrotron radiation · 2024Article
- Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications.International journal of molecular sciences · 2024Article
- Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy.Cells · 2022Article
- Demonstration of Near Edge X-ray Absorption Fine Structure Spectroscopy of Transition Metals Using Xe/He Double Stream Gas Puff Target Soft X-ray Source.Materials (Basel, Switzerland) · 2021Article
- Quantitative conversion of biomass in giant DNA virus infection.Scientific reports · 2021Article
- X-ray tomography of cryopreserved human prostate cancer cells: mitochondrial targeting by an organoiridium photosensitiser.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography.Plant methods · 2020Article
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- Perspective: Towards single shot time-resolved microscopy using short wavelength table-top light sources.Structural dynamics (Melville, N.Y.) · 2019Article
- Dynamic Tomographic Reconstruction of Deforming Volumes.Materials (Basel, Switzerland) · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water-window x-ray microscopy allows two- and three-dimensional (2D and 3D) imaging of intact unstained cells in their cryofixed near-native state with unique contrast and high resolution. Present operational biological water-window microscopes are based at synchrotron facilities, which limits their accessibility and integration with complementary methods. Laboratory-source microscopes have had difficulty addressing relevant biological tasks with proper resolution and contrast due to long exposure times and limited up-time. Here we report on laboratory cryo x-ray microscopy with the exposure time, contrast, and reliability to allow for routine high-spatial resolution 3D imaging of intact cells and cell-cell interactions. Stabilization of the laser-plasma source combined with new optics and sample preparation provide high-resolution cell imaging, both in 2D with ten-second exposures and in 3D with twenty-minute tomography. Examples include monitoring of the distribution of carbon-dense vesicles in starving HEK293T cells and imaging the interaction between natural killer cells and target cells.
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