ArticleScientific reports2017
Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 79 citations in OpenAlex.
- The morphogenetic activity of dAnk genes in the diatom Thalassiosira pseudonana is sensitive to Si availability.Communications biology · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Increased aluminum exposure induces widespread changes in silicon, carbon, and nitrogen metabolism in Entomoneis vertebralis.BMC genomics · 2025Article
- Characterization of two proline-rich proteins involved in silicon deposition inFrontiers in plant science · 2025Article
- The draft genome of Nitzschia closterium f. minutissima and transcriptome analysis reveals novel insights into diatom biosilicification.BMC genomics · 2024Article
- Nanoengineered Silica-Based Biomaterials for Regenerative Medicine.International journal of molecular sciences · 2024Review
- Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation.Biotechnology for biofuels and bioproducts · 2023Article
- Exocytosis of the silicified cell wall of diatoms involves extensive membrane disintegration.Nature communications · 2023Article
- The molecular basis for pore pattern morphogenesis in diatom silica.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Geometrical frustration of phase-separated domains inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural evidence for extracellular silica formation by diatoms.Nature communications · 2021Article
- Identification of the Domains Involved in Promotion of Silica Formation in Glassin, a Protein Occluded in Hexactinellid Sponge Biosilica, for Development of a Tag for Purification and Immobilization of Recombinant Proteins.Marine biotechnology (New York, N.Y.) · 2020Article
- Cloning ofBiology · 2020Article
- Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases.Marine biotechnology (New York, N.Y.) · 2020Article
- Control of biosilica morphology and mechanical performance by the conserved diatom geneCommunications biology · 2019Article
- Anomalies in the valve morphogenesis of the centric diatom algaBiology open · 2018Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diatoms are known for their intricate, silicified cell walls (frustules). Silica polymerization occurs in a compartment called the silica deposition vesicle (SDV) and it was proposed that the cytoskeleton influences silica patterning through the SDV membrane (silicalemma) via interactions with transmembrane proteins. In this work we identify a family of proteins associated with the silicalemma, named SAPs for Silicalemma Associated Proteins. The T. pseudonana SAPs (TpSAPs) are characterized by their motif organization; each contains a transmembrane domain, serine rich region and a conserved cytoplasmic domain. Fluorescent tagging demonstrated that two of the TpSAPs were localized to the silicalemma and that the intralumenal region of TpSAP3 remained embedded in the silica while the cytoplasmic region was cleaved. Knockdown lines of TpSAP1 and 3 displayed malformed valves; which confirmed their roles in frustule morphogenesis. This study provides the first demonstration of altering silica structure through manipulation of a single gene.
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